NF-kB p50/p65 Transcription Factor Assay Kit
| Name | NF-kB p50/p65 Transcription Factor Assay Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab133128 |
| Category | Cellular Assay |
| Prices | $494.00 |
| Sizes | 96 tests |
| Assay Type | Indirect |
| Sample Type | Cell culture extracts, Tissue Extracts, Nuclear Extracts |
| Applications | FA |
| Species Reactivities | Mouse, Rat, Human |
| Gene | RELA |
| Supplier Page | Shop |
Product images
Jurkat cells were treated with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116). 50 uL of the cytosolic fraction (ab113474), corresponding to 1e6 cells, were tested.
Jurkat cells were treated for 24 hours with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116). Nuclear lysates were extracted (ab113474) and 40 uL, corresponding to 4e6 cells, were tested.
Titration of positive control with or without transcription factor NF-kB competitor dsDNA (inhibitor), background signal subtracted.
Assay of cell lysates isolated from stimulated (20 ng/ml TNF-alpha for 30 min) and non-stimulated HeLa cells demonstrating NF-kB p65 activity.
Assay of human NF-kB p50 from the nuclear fraction of TNF-alpha activated HeLa cells.
Product References
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Godbersen JC, Humphries LA, Danilova OV, Kebbekus PE, Brown JR, Eastman A, Danilov AV. Clin Cancer Res. 2014 Mar 15;20(6):1576-89.
The primary cilium influences interleukin-1beta-induced NFkappaB signalling by - The primary cilium influences interleukin-1beta-induced NFkappaB signalling by
Wann AK, Chapple JP, Knight MM. Cell Signal. 2014 Aug;26(8):1735-42.