Translocation of SREBP-2 into nuclei by U-18666A. Raw 264.7 cells were seeded in a 96-well plate at a density of 3 x 104 cells/well and cultured overnight. The next day, cells were treated with DMSO (vehicle) or 24 µM U-18666A for 72 hours. Panel A: Cells treated with DMSO alone demonstrate cytoplasmic localization of SREBP-2, indicating that most of cells have inactive protein. Panel B: U-18666A treatment for three days induced SREBP-2 translocation into the nuclei, indicating that blockage of cholesterol transport in these cells activates the protein.