Figure 2. The relationship between the protein concentration of lysates from untreated, phosphatase-treated and H 2 O 2 -treated C2C12 cells and the absorbance at 450 nm using the PathScan ® Phospho-AMPKα (Thr172) Sandwich ELISA Kit is shown.
Figure 1. Treatment of C2C12 cells with H 2 O 2 stimulates phosphorylation of AMPKα at Thr172, detected by the PathScan ® Phospho-AMPKα (Thr172) Sandwich ELISA Kit #7959. C2C12 cells (80-90% confluent) were treated with 10 mM H 2 O 2 for 10 minutes at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using AMPKα (23A3) Rabbit mAb #2603 (left panel) or Phospho-AMPKα (Thr172) (D79.5E) XP ® Rabbit mAb #4188 (right panel) are shown in the bottom figure.