PathScan ® Total SQSTM1/p62 Sandwich ELISA Kit
| Name | PathScan ® Total SQSTM1/p62 Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7814 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human, Mouse, Rat, Monkey, Mink |
| Description | The PathScan ® Total SQSTM1/p62 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total SQSTM1/p62 protein |
| Gene | ATG4A |
| Supplier Page | Shop |
Product images
Figure 1. SQSTM1/p62 protein from SK-MEL-2 cells is detected using PathScan ® Total SQSTM1/p62 Sandwich ELISA Kit #7814. This kit detects lower levels of SQSTM1/p62 in SK-MEL-2 cells starved overnight in Earle's Balanced Salt Solution (EBSS). The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using SQSTM1/p62 (D5E2) Rabbit mAb #8025 is shown in the bottom figure.
Figure 2. The relationship between protein concentration of lysates from unstarved and EBSS-starved SK-MEL-2 cells and the absorbance at 450 nm is shown. SK-MEL-2 cells (85% confluence) were starved overnight in EBSS at 37ºC and then lysed.