PathScan ® Phospho-Bad (Ser112) Sandwich ELISA Kit
| Name | PathScan ® Phospho-Bad (Ser112) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7182 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human, Mouse, Monkey |
| Description | CST's PathScan ® Phospho-Bad (Ser112) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-Bad (Ser112) protein |
| Gene | BAD |
| Supplier Page | Shop |
Product images
Figure 1. Treatment of OVCAR8 cells with TPA stimulates phosphorylation of Bad at Ser112, detected by PathScan ® Phospho-Bad (Ser112) Sandwich ELISA Kit #7182, but does not affect the level of total Bad protein detected by PathScan ® Total Bad Sandwich ELISA Kit #7162. Lambda phosphatase treatment of control cell lysates (4000 U/mL for 60 minutes at 37ºC) abolishes the basal phosphorylation of Bad as shown by both Sandwich ELISA and Western analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blots using Phospho-Bad (Ser112) Antibody #9296 (right panel) or Bad Antibody #9254 (left panel), are shown in the bottom figure.
Figure 2. The relationship between the protein concentration of lysates from untreated and TPA-treated OVCAR8 cells and the absorbance at 450 nm is shown. Cells were serum starved overnight and then treated with 200 nm TPA for 30 min. at 37ºC.