Figure 1: Treatment of HeLa cells with staurosporine stimulates cleavage of caspase-3 protein, detected by PathScan® Cleaved Caspase-3 (Asp175) Sandwich ELISA Kit #7190 (top figure), but does not affect the level of total caspase-3 protein detected by western blot (bottom figure), using Cleaved Caspase-3 (Asp175) Antibody #9661 (upper panel) or Caspase-3 Antibody #9662 (lower panel).
Figure 2: Linear relationship between protein concentration of staurosporine-treated HeLa cell lysates and kit assay optical density readings. HeLa cells (90% confluence) were treated with staurosporine (1 uM), and lysed after incubation at 37ºC for 3 hours.