HTScan® Mouse PDGF Receptor α Kinase Assay Kit

• Supplier: Cell Signaling Technology
• Catalog: 7919
• Category: Assay Kit
• Species Reactivities: Mouse
• Description: The kit provides a means of performing kinase activity assays with recombinant mouse PDGFRalpha kinase
• Gene: PDGFRA

Product images

Figure 1. Mouse PDGFRalpha kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, 100 µM ATP, 250 µM FLT3 (Tyr589) biotinylated peptide and variable amount of Recombinant mouse PDGFRalpha. Reaction mixture incubated at room temperature for 10 minutes.

Figure 3. Dose dependence curve of mouse PDGFRalpha kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of substrate peptide (#1305) by mouse PDGFRalpha kinase. In a 50 µl reaction, increasing amounts of mouse PDGFRalpha and 1.5 µM substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Figure 5. Staurosporine inhibition of mouse PDGFRalpha kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of PDGFRalpha substrate peptide (#1305) by the mouse PDGFRalpha kinase. In a 50 µl reaction, 60 ng PDGFRalpha, 1.5 µM substrate peptide, 20 µM ATP and increasing amounts of staurosporine were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Figure 4. Peptide concentration dependence of mouse PDGFRalpha kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of substrate peptide (#1305) by the mouse PDGFRalpha kinase. In a 50 µl reaction, 60 ng of PDGFRalpha and increasing concentrations of substrate peptide were used per reaction at room temperature for 30 minutes. (DELFIA® is a registered trademark of PerkinElmer, Inc.)

Figure 2. Time course of mouse PDGFRalpha kinase activity: DELFIA® data generated using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 to detect phosphorylation of PDGFRalpha substrate peptide (#1305) by the mouse PDGFRalpha kinase. In a 50 µl reaction, 60 ng PDGFRalpha and 1.5 µM substrate peptide were used per reaction. (DELFIA® is a registered trademark of PerkinElmer, Inc.)