PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit
| Name | PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7176 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human |
| Description | CST's PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-cdc2 (Tyr15) protein |
| Gene | CDK1 |
| Supplier Page | Shop |
Product images
Figure 2: The relationship between protein concentration of lysates from lambda phosphatase and IL-4 treated HeLa cells and kit assay optical density reading. HeLa cells (80% confluent) were treated with human IL-4 for 10 minutes (100 ng/ml).
Figure 1: Treatment of HeLa cells with Interleukin-4 stimulates phosphorylation of cdc2 at Tyr15 as detected by PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA kit #7176, but does not affect the level of total cdc2 protein detected by cdc2 Antibody (#9116). OD 450 readings are shown in the top figure, while the corresponding Western blots using Phospho-cdc2 (Tyr15) Antibody #9111 (left panel) or cdc2 Antibody #9116 (right panel) is shown in the bottom figure.