HTScan® PKCθ Kinase Assay Kit
| Name | HTScan® PKCθ Kinase Assay Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7606 |
| Category | Assay Kit |
| Description | The kit provides a means of performing kinase activity assays with recombinant human PKCtheta kinase |
| Gene | PRKCQ |
| Supplier Page | Shop |
Product images
Figure 1. PKCtheta kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 5 mM MgCl2, 1.32 mM CaCl2, 1 mM EDTA, 1.25 mM EGTA, 0.25 μg/50 μl Phosphatidylserine, 50 ng/50 μl 1.2 Dioleyl-glycerol, 1.2 mM DTT, ATP (variable), 2.5 μg/50 μl PEG 20.000, Substrate: Histone H2B, 2 μg/50 μl and recombinant PKCtheta: 200 ng/50 µl.