ARG20524 anti-eNOS phospho (Ser1177) antibody WB image Western Blot: 1) and 2) calyculin A (100 nM) treated human umbilical vein endothelial cells for 30 min; 2) then the blots were treated with lambda phosphatase. The blots were stained with ARG20524 anti-eNOS phospho (Ser1177) antibody.