HeLa cells were treated with FBS. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
293 cells were treated or untreated with Insulin. Cell lysates were analyzed using this phosphoELISA and Western Blot.