EIF4EBP1

Gene Symbol EIF4EBP1
Entrez Gene 1978
Alt Symbol 4E-BP1, 4EBP1, BP-1, PHAS-I
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 4E binding protein 1
Other Description eIF4E-binding protein 1|eukaryotic translation initiation factor 4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
Swissprots Q6IBN3 Q13541 B2R502 D3DSW8
Accessions CCW44094 EAW63341 EAW63342 Q13541 AB044548 BAB18650 AK312011 BAG34949 BC004459 AAH04459 BC058073 AAH58073 BF964628 BT007162 AAP35826 CR456769 CAG33050 DQ890570 ABM92187 DQ893731 ABM84657 L36055 AAA62269 NM_004095 NP_004086
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. {ECO:0000269|PubMed:22578813, ECO:0000269|PubMed:7935836}.
Top Pathways RNA transport, Choline metabolism in cancer, AMPK signaling pathway, PI3K-Akt signaling pathway, ErbB signaling pathway

PathScan ® Phospho-4E-BP1 (Ser65) Sandwich ELISA Kit - 13777 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of HEK-293T cells with amino acids and insulin stimulates phosphorylation of 4E-BP1 at Ser65, as detected by PathScan ® Phospho-4E-BP1 (Ser65) Sandwich ELISA kit #13777, but does not affect the level of total 4E-BP1 protein detected by western analysis. HEK-293T cells (70-80% confluent) were starved overnight and deprived of amino acids for 1 hr. The amino acids were replenished for 1 hr. Cells were either untreated (-) or stimulated with 100 nM insulin for 30 min at 37 o C (+). Treatment of control cell lysates with λ phosphatase (4000 U/ml, 60 min, 37 o C) abolishes the basal phosphorylation of 4E-BP1 as shown by both sandwich ELISA and western blot analysis. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots, using 4E-BP1 (53H11) Rabbit mAb #9644 (left panel) or Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb #9456 (right panel), are shown in the bottom figure.

PathScan ® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Kit - 7216 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of HEK-293T cells with amino acids and insulin stimulates phosphorylation of 4E-BP1 at Thr37/46, as detected by PathScan ® Phospho-4E-BP1 (Thr37/46) Sandwich ELISA Kit #7216, but does not affect the level of total 4E-BP1 protein detected by Western analysis. HEK-293T cells (70-80% confluent) were starved overnight and deprived of amino acids for 1 hour. The amino acids were replenished for 1 hour. Cells were either untreated or stimulated with 100 nM insulin for 30 minutes at 37ºC. λ phosphatase treatement of control cell lysates (4000 U/mL for 60 minutes at 37ºC) abolishes the basal phosphorylation of 4E-BP1 as shown by both sandwich ELISA and Western analysis. The absorbance readings at 450 nM are shown in the top figure, while the corresponding Western blots, using 4E-BP1 Antibody #9452 (left panel) or Phospho-4E-BP1 (Thr37/46) Antibody #9459 (right panel), are shown in the bottom figure.

PathScan ® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Antibody Pair - 7854 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between the protein concentration of lysate from amino acid (AA)/untreated and AA/insulin-treated HEK- 293T cells and the absorbance at 450 nM using PathScan ® Phospho-4E-BP1 (Thr37/Thr46) Sandwich ELISA Antibody Pair #7854 is shown. HEK-293T cells were serum-starved overnight and deprived of amino acids for 1 hour. The amino acids were replenished for 1 hour. Cells were either untreated or stimulated with 100 nM insulin for 30 minutes at 37ºC.

PathScan ® Total 4E-BP1 Sandwich ELISA Kit - 7179 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of HEK-293T cells with amino acids and insulin stimulates phosphorylation of 4E-BP1 at Thr37/46, detected by PathScan ® Phospho-4E-BP1 (Thr37/46) Sandwich ELISA Kit #7216, but does not affect the level of total 4E-BP1 protein detected by PathScan ® Total 4E-BP1 Sandwich ELISA Kit #7179. HEK-293T cells (70-80% confluent) were starved overnight and deprived of amino acids for 1 hour. The amino acids were replenished for 1 hour. Cells were either untreated or stimulated with 100 nM insulin for 30 minutes at 37ºC. λ phosphatase treatment of control cell lysates (4000 U/mL for 60 minutes at 37ºC) abolishes the basal phosphorylation of 4E-BP1 as shown by both sandwich ELISA and Western analysis. The absorbance readings at 450 nm are shown in the top figure while the corresponding Western blots, using 4E-BP1 Antibody #9452 (left panel) or Phospho-4E-BP1 (Thr37/46) Antibody #9459 (right panel), are shown in the bottom figure.

Mouse/Human/Rat Phospho-EIF4EBP1 / 4EBP1 (Ser64) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1112 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Rat Phospho-EIF4EBP1 / 4EBP1 (Thr69) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1113 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human/Rat EIF4EBP1 / 4EBP1 ELISA Kit (Cell-Based ELISA) - LS-F1770 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate

Mouse/Human/Rat Phospho-EIF4EBP1 / 4EBP1 (Thr36) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1445 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Mouse/Human/Rat Phospho-EIF4EBP1 / 4EBP1 (Thr45) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1446 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate

Eukaryotic translation initiation factor 4E-binding protein 1 (EIF4EBP1), Human, ELISA Kit - CSB-EL007561HU from ARP American Research Products

Category ELISA Kit
Prices $495.00, $695.00, $696.00, $699.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t

Anserine P-P38MAPK ELISA Kit - AP0030 from NeoScientific

Category ELISA Kit
Prices $300.00, $480.00
Sizes 100 µl, 200 µl

4E-BP1 Colorimetric Cell-Based ELISA Kit - CB5008 from Assay Biotechnology

Category ELISA Kit
Prices $380.00, $760.00, $1,140.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

4E-BP1 (Phospho-Ser64) Colorimetric Cell-Based ELISA Kit - CBP1093 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

4E-BP1 (Phospho-Thr36) Colorimetric Cell-Based ELISA Kit - CBP1487 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

4E-BP1 (Phospho-Thr45) Colorimetric Cell-Based ELISA Kit - CBP1488 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

eukaryotic translation initiation factor 4E binding protein 1, ELISA Kit - MBS934012 from MyBioSource

Category ELISA Kit
Prices $250.00, $495.00, $710.00, $2,705.00, $5,145.00
Sizes 24 -strip-wells (Limit 1), 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity The minimum detectable dose of human EIF4EBP1 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Range 46.88 pg/ml -3000 pg/ml.

Human Eukaryotic translation initiation factor 4E-binding protein 1(EIF4EBP1) EL - CSB-EL007561HU from Cusabio

Sensitivity 11.72 pg/ml
Range 46.88 pg/ml-3000 pg/ml

Human/Mouse Phospho-4E-BP1 (T36) ELISA - PEL-4EBP1-T36 from RayBiotech

Category ELISA Kit
Prices $481.00, $866.00, $1,924.00
Sizes 1 plate kit, 2 plate kit, 5 plate kit
4E-BP1 (Thr36)- Positive Control

Human/Mouse Phospho-4E-BP1 (T36) and Total 4E-BP1 ELISA - PEL-4EBP1-T36-T from RayBiotech

Category ELISA Kit
Prices $525.00, $946.00, $2,101.00
Sizes 1 plate kit, 2 plate kit, 5 plate kit
4E-BP1 (Thr36)- Positive Control

4E-BP1 ELISA Kit - DEIA-XYA137 from Creative Diagnostics

Category ELISA Kit

4E-BP1 (Phospho-Ser64) ELISA Kit - DEIA-XYA138 from Creative Diagnostics

Category ELISA Kit

4E-BP1 (Phospho-Thr36) ELISA Kit - DEIA-XYA139 from Creative Diagnostics

Category ELISA Kit

4E-BP1 (Phospho-Thr45) ELISA Kit - DEIA-XYA140 from Creative Diagnostics

Category ELISA Kit

Human/Mouse 4E-BP1 (Phospho-T36) ELISA Kit - orb320091 from Biorbyt

Category ELISA Kit
Prices $918.00, $1,573.00, $3,366.00
Sizes 96 assays, 192 assays, 480 assays

Human/Mouse Phospho-4E-BP1 (T36) ELISA - PEL-4EBP1-T36-1 from RayBiotech

Category ELISA Kit
Prices $491.00, $883.80, $1,964.00
Sizes 1 plate kit, 2 plate kit, 5 plate kit
HeLa cells were treated with FBS. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
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