T47D cells were treated with IGF-1 at 37°C for 30 min. Solubilize cells at 4 x 10^7 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
HeLa cells were treated or untreated with Insulin (1µM) for 5 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.
HeLa cells were treated or untreated with Insulin (1µM) for 5 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.