AKT1S1

Gene Symbol	AKT1S1
Entrez Gene	84335
Alt Symbol	Lobe, PRAS40
Species	Human
Gene Type	protein-coding
Description	AKT1 substrate 1 (proline-rich)
Other Description	40 kDa proline-rich AKT substrate|proline-rich AKT1 substrate 1
Swissprots	A8MTQ1 Q96B36 Q9BWR5 Q96BI4 J3KPM3 B2RE93 Q96NG2 Q96IK7
Accessions	EAW52568 EAW52569 EAW52570 EAW52571 Q96B36 AK055511 BAB70937 AK092610 BAG52583 AK123687 AK226004 AK316603 BAG38190 BC000031 AAH00031 BC007416 AAH07416 BC015562 AAH15562 BC016043 AAH16043 BC022241 BC051844 AAH51844 DA005259 DA476468 DA542314 DA848626 NM_001098632 NP_001092102 NM_001098633 NP_001092103 NM_001278159 NP_001265088 NM_001278160 NP_001265089 NM_032375 NP_115751
Function	Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phospho
Subcellular Location	Cytoplasm, cytosol {ECO:0000250}. Note=Found in the cytosolic fraction of the brain. {ECO:0000250}.
Tissue Specificity	Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). {ECO:0000269|PubMed:12524439, ECO:0000269|PubMed:16174443}.
Top Pathways	AMPK signaling pathway, mTOR signaling pathway

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

PathScan ® Phospho-PRAS40 (Thr246) Sandwich ELISA Kit - 7327 from Cell Signaling Technology Category ELISA Kit Prices $489.00 Sizes 1 kit (96 assays)

PathScan ® Total PRAS40 Sandwich ELISA Kit - 7331 from Cell Signaling Technology Category ELISA Kit Prices $489.00 Sizes 1 kit (96 assays)

Mouse/Human/Rat Phospho-AKT1S1 / PRAS40 (Thr246) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1152 from LifeSpan Bioscience Category ELISA Kit Prices $550.00 Sizes 1 plate

Mouse/Human/Rat AKT1S1 / PRAS40 ELISA Kit (Cell-Based ELISA) - LS-F1787 from LifeSpan Bioscience Category ELISA Kit Prices $450.00 Sizes 1 plate

Phospho-PRAS40 (T246), Human/Mouse, BioAssay™ Cell-Based ELISA (AKT1S1, Lobe) - 145760 from USBiological Life Science

Human Phospho-PRAS40 (T246) DuoSet IC ELISA, 2 Plate - DYC6890-2 from R&D Systems Category ELISA Kit Prices $329.00 Sizes 1 kit (for 2 Plates) Range 937.50 - 60,000 pg/mL

Human/Mouse Phospho-PRAS40 (T246) Cell-Based ELISA - KCB6890 from R&D Systems Category ELISA Kit Prices $459.00 Sizes 1 kit

Proline-rich AKT1 substrate 1, AKT1S1, Human, ELISA Kit - E14177h from ARP American Research Products Category ELISA Kit Prices $476.00 Sizes 96 t

Human Phospho-PRAS40 (T246) ELISA - PEL-PRAS40-T246 from RayBiotech Category ELISA Kit Prices $481.00, $866.00, $1,924.00 Sizes 1 plate kit, 2 plate kit, 5 plate kit

Akt1 S1 Colorimetric Cell-Based ELISA Kit - CB5029 from Assay Biotechnology Category ELISA Kit Prices $380.00, $760.00, $1,140.00 Sizes 1 kit, 2 kits, 3 kits Range > 5000 Cells

Akt1 S1 (Phospho-Thr246) Colorimetric Cell-Based ELISA Kit - CBP1137 from Assay Biotechnology Category ELISA Kit Prices $480.00, $960.00, $1,440.00 Sizes 1 kit, 2 kits, 3 kits Range > 5000 Cells

AKT1 substrate 1 (proline-rich) , ELISA Kit - MBS9316442 from MyBioSource Category ELISA Kit Prices $435.00, $600.00, $2,650.00, $5,205.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells Sensitivity 5.0pg/ml. Range 31.2pg/ml-1000pg/ml.

PRAS40 ELISA Kit - DEIA7242 from Creative Diagnostics Category ELISA Kit Sensitivity Sensitivity Notes: The analytical sensitivity of this assay is Figure 1. The sensitivity of this ELISA w Range 0.313-20 ng/mL

phospho-PRAS40 (Thr246) STAR ELISA Kit - 17-478 from EMD Millipore Category ELISA Kit Sensitivity Sensitivity: 1 unit/mL Range of Detection: 1.6 to 100 units/mL Species Reactivity: Human

PRAS40 STAR ELISA Kit - 17-477 from EMD Millipore Category ELISA Kit Sensitivity Sensitivity: 15.6 pg/mL Range of Detection: 31.2 to 2000 pg/mL

Mouse AKT1S1 / PRAS40 ELISA Kit (Custom ELISA) - LS-F17185 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity To be determined Range To be determined

Human AKT1S1 / PRAS40 ELISA Kit (Custom ELISA) - LS-F17186 from LifeSpan Bioscience Category ELISA Kit Prices $530.00 Sizes 1 plate Sensitivity To be determined Range To be determined

Human PRAS40 (Phospho-T246) ELISA Kit - orb247229 from Biorbyt Category ELISA Kit Prices $995.00, $1,709.00, $3,672.00 Sizes 96 assays, 192 assays, 480 assays

Human PRAS40 (Phospho-T246) ELISA Kit - orb247221 from Biorbyt Category ELISA Kit Prices $995.00, $1,709.00, $3,672.00 Sizes 96 assays, 192 assays, 480 assays

Human Phospho-PRAS40 (T246) ELISA - PEL-PRAS40-T246-1 from RayBiotech Category ELISA Kit Prices $481.00, $865.80, $1,924.00 Sizes 1 plate kit, 2 plate kit, 5 plate kit

Human Phospho-PRAS40 (T246) & Total PRAS40 ELISA - PEL-PRAS40-T246-T-1 from RayBiotech Category ELISA Kit Prices $525.00, $945.00, $2,100.00 Sizes 1 plate kit, 2 plate kit, 5 plate kit

Human AKT1S1 ELISA Kit - E074987 from Biobool Category ELISA Kit

Proline-rich AKT1 substrate 1, ELISA Kit - MBS2882882 from MyBioSource Category ELISA Kit Prices $380.00, $500.00, $2,170.00, $3,840.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells Range 0.15 ng/mL -10.0 ng/mL

Proline-Rich AKT1 Substrate 1 (AKT1S1), ELISA Kit - MBS9377129 from MyBioSource Category ELISA Kit Prices $435.00, $600.00, $2,650.00, $5,205.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Akt1 S1 Colorimetric Cell-Based ELISA Kit - EKA50022 from Biomatik Category ELISA Kit Prices $399.00 Sizes 96 tests Sensitivity At least 5000 cells/well Range Qualitative

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