Background-subtracted data values (mean +/- SD) are graphed.
Non-background subtracted data from duplicate measurements are plotted. CA125 was detectable in HeLa cells culture supernatants (4-days culture), HepG2 and THP1 cell culture supernatants (4-days culture) were loaded as negative controls.
Duplicate interpolated values of CA125 (mean +/- SD, n = 2) are graphed. Medical histories of individual donors was not known. Measurements of CA125 are known to fluctuate in individual serums due to onset of certain cancers and benign conditions, thus CA125 elevation alone is not reason enough for diagnosis.