Background-subtracted data values (mean +/- SD) are graphed.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Clusterin was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Clusterin Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 1.5 ng/mL unstimulated, 16.4 ng/mL stimulated. There was no detectable signal in media.
Interpolated concentrations of Clusterin in mouse serum and plasma (citrate). The concentrations of Clusterin were measured in duplicate and interpolated from the Clusterin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 59.1 µg/mL in mouse serum and 38.7 µg /mL in mouse plasma.