MAFF

Gene Symbol	MAFF
Entrez Gene	23764
Alt Symbol	U-MAF, hMafF
Species	Human
Gene Type	protein-coding
Description	v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F
Other Description	transcription factor MafF\|v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F
Swissprots	Q9ULX9 Q9Y525 B4DV49
Accessions	EAW60221 Q9ULX9 AB025247 BAA86871 AI610651 AJ010857 CAB52435 AK002001 BAA92029 AK097953 AK300932 BAG62561 BC015037 AAH15037 BC067751 AAH67751 DA209378 DA425613 DA654696 DB319587 NM_001161572 NP_001155044 NM_001161573 NP_001155045 NM_001161574 NP_001155046 NM_012323 NP_036455 NM_152878
Function	Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response.
Subcellular Location	Nucleus.
Tissue Specificity	Expressed in the term myometrium and kidney.

MafF/G/K (D-12) - sc-166548 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

MafF/G/K (D-12): sc-166548. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.

Anti-MAFF antibody - ab198249 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling MAFF with ab198249 at 1/20 dilution.

Anti-MAFF antibody - ab91486 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF antibody (ab91486) at 1/50 dilution + Mouse liver tissue lysates at 35 µg

Anti-MAFF antibody - ab110824 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab110824, at 1/50 dilution, staining MAFF in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-MAFF antibody - ab183859 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-MAFF antibody (ab183859) at 1/2000 dilutionLane 1 : Huh7 whole cell lysate/extractLane 2 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.

Anti-MAFF Antibody (N-Terminus) - LS-C25683 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF Antibody (aa51-100) - LS-C119325 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF Antibody (aa33-82) - LS-C31521 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF Antibody (aa66-92) - LS-C163967 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin-embedded human skin reacted with MAFF Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

MafF Antibody [Unconjugated] - AF3917 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot MafF Antibody [Unconjugated]

MafF Antibody - NBP1-55753 from Novus Biologicals

Prices	$229.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: MAFF Antibody [NBP1-55753] - Analysis of paraffin-embedded human breast carcinoma tissue, using MAFF Antibody. The picture on the right is treated with the synthesized peptide.

MafF Antibody (1F8) - H00023764-M02-100ug from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F8

Anti-MAFF (N-terminal) antibody produced in rabbit - M8569 from Sigma-Aldrich

Prices	$428.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Whole cell lysates of A431 was separated on SDS-PAGE and probed with Anti-MAFF (N-terminal) (<B>Cat. No. M8569</B>). The antibody was developed with Anti-Rabbit IgG, Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1: Antibody dilution 1:4,000<BR/>2: Antibody dilution 1:8,000<BR/>3: Antibody dilution 1:4,000 + 10 μg/mL MAFF immunizing peptide<BR/>4: Without primary antibody

Anti-MAFF (C-Terminal) antibody produced in rabbit - M8194 from Sigma-Aldrich

Prices	$428.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoprecipitation</B><BR/>(Lanes 2, 3, 4) 2, 5, and 10 μL of Rabbit Anti-MAFF (C-terminal) (<B>Cat. No. M8194</B>) respectively were used to immunoprecipitate human MAFF from 0.5 mg of A431 cell lysate. Detection antibody: Rabbit Anti-MAFF (N-terminal) (<B>Cat. No. M8569</B>). MAFF immunoprecipitates at ~18 kDa. (Lane 1) Negative control — without cell lysate; (Lane 5) Negative control — without IP antibody.

Monoclonal Anti-MAFF antibody produced in mouse - SAB1412819 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F8

<B>Immunoblotting</B><BR/>Anti-MAFF:<B>Cat. No. SAB1412819</B>: Antibody concentration: 15 μg/mL. Immunofluorescence band of ~18 kDa using Hela NE lysate.

Anti-MAFF antibody produced in rabbit - SAB4501028 from Sigma-Aldrich

Prices	$335.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-MAFF: <B>Cat. No. SAB4501028</B>: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-MAFF antibody. Cells on the left were treated with the Anti-MAFF antibody. Cells on the right (negative control) were treated with both Anti-MAFF antibody and the synthesized immunogen peptide.

Anti-MAFF antibody produced in rabbit - AV38984 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV38984</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 5 μg/mL in cell type HepG2

MAFF antibody - N-terminal region (ARP38984_T100) - ARP38984_T100 from Aviva Systems Biology

Prices	$229.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

MAFF-Antibody-Center - AP6837c from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	80 µl, 400 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin-embedded human skin reacted with MAFF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

MAFF Antibody - middle region (OAAB03938) - OAAB03938 from Aviva Systems Biology

Prices	$289.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

MAFF antibody - GTX87425 from GeneTex

Prices	$319.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX87425

MAFF antibody - GTX120264 from GeneTex

Prices	$119.00
Sizes	25 μl
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF antibody used in Western blot. GTX120264

MAFF antibody - GTX129249 from GeneTex

Prices	$119.00
Sizes	25 μl
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF antibody used in Western blot. GTX129249

MAFF antibody, Internal - GTX81696 from GeneTex

Prices	$319.00
Sizes	400 μl
Host	Rabbit
Clonality	Polyclonal

Anti-MAFF antibody, Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX81696

Human MafF Antibody - AF3917 from R&D Systems

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat

Detection of Human MafF by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MafF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band for MafF was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

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