GBA

Gene Symbol GBA
Entrez Gene 537087
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description glucosidase, beta, acid
Other Description D-glucosyl-N-acylsphingosine glucohydrolase|acid beta-glucosidase|beta-glucocerebrosidase|glucosidase, beta; acid (includes glucosylceramidase)|glucosylceramidase
Swissprots Q2KHZ8
Accessions DAA31806 Q2KHZ8 BC112823 AAI12824 XM_005203735 XP_005203792 XR_805328 XR_805329 NM_001046421 NP_001039886
Subcellular Location Lysosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2. {ECO:0000250}.
Top Pathways Lysosome, Sphingolipid metabolism, Other glycan degradation

Anti-GBA antibody - ab92997 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
GBA was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to GBA and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab92997.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 60kDa; GBA

Anti-GBA antibody - ab175869 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GBA antibody (ab175869) + Mouse Lung Lysate at 35 µgdeveloped using the ECL techniqueObserved band size : 56 kDa (why is the actual band size different from the predicted?)

Anti-GBA antibody - ab96246 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-GBA antibody (ab96246) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

Anti-GBA antibody - ab96256 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-GBA antibody (ab96256) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-GBA antibody - ab118304 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GBA antibody (ab118304) at 1 µg/ml + MDA-MB-435S cell lysate at 10 µg

Anti-GBA antibody [1D8] - ab180537 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D8
All lanes : Anti-GBA antibody [1D8] (ab180537) at 1/2000 dilutionLane 1 : Lysate from HEK293T cells transfected with pCMV6-ENTRY control cDNA for 48 hrsLane 2 : Lysate from HEK293T cells transfected with pCMV6-ENTRY GBA cDNA for 48 hrsLysates/proteins at 5 µg per lane.developed using the ECL technique
Search more