SEMA4F

Gene Symbol SEMA4F
Entrez Gene 10505
Alt Symbol M-SEMA, PRO2353, S4F, SEMAM, SEMAW, m-Sema-M
Species Human
Gene Type protein-coding
Description sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
Other Description semaphorin M|semaphorin-4F|semaphorin-M|semaphorin-W
Swissprots Q542Y7 O95754 Q9NS35
Accessions CAI72085 EAW99605 EAW99606 EAW99607 EAW99608 O95754 AB021292 AB022317 BAA75631 AF053369 AAF80660 AK075384 BAC11584 AK095587 AK291023 BAF83712 AK304358 BAH14167 AK308166 AK312728 AL136552 CAB66487 AM393735 CAL38610 BC018361 AAH18361 BC038411 AAH38411 CK905501 DC306380 DQ895677 ABM86603 XM_011532475 XP_011530777 XM_011532476 XP_011530778 XM_011532477 XP_011530779 XR_939650 NM_001271661 NP_001258590 NM_001271662 NP_001258591 NM_004263 NP_004254 NR_073398 NR_073399
Function Has growth cone collapse activity against retinal ganglion-cell axons. {ECO:0000250}.
Subcellular Location Membrane; Single-pass type I membrane protein.
Top Pathways Axon guidance

SEMA4F (H-138) - sc-135264 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SEMA4F (H-138): sc-135264. Western blot analysis of SEMA4F expression in A-431 whole cell lysate.

SEMA4F (N-20) - sc-67946 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SEMA4F (V-19) - sc-67948 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-SEMA4F antibody - ab86549 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SEMA4F antibody (ab86549) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-SEMA4F / Semaphorin 4F Antibody (aa91-140) - LS-C111097 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SEMA4F antibody LS-C111097 Western blot of Transfected 293T cell lysate.

Anti-SEMA4F / Semaphorin 4F Antibody (aa21-70) - LS-C102228 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SEMA4F antibody LS-C102228 Western blot of Transfected 293T cell lysate.

Anti-SEMA4F / Semaphorin 4F Antibody (aa695-709) - LS-C344254 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from A549, U-87 MG cell line and rat lung tissue lysate (from left to right) with SEMA4F Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Semaphorin 4F Antibody (780225) - MAB7200 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Rat
Clonality Monoclonal
Clone 780225
Semaphorin 4F was detected in perfusion fixed frozen sections of mouse spinal cord using Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to synaptic puncta. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Semaphorin 4F Antibody - AF7200 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows recombinant mouse Semaphorin 4F. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4F Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7200) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for recombinant mouse Semaphorin 4F at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Semaphorin 4F Antibody - NBP1-59942 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SEMA4F Antibody [NBP1-59942] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.

Semaphorin 4F Antibody - H00010505-B01P-50ug from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

SEMA4F Polyclonal Antibody - bs-11478R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-SEMA4F Polyclonal Antibody, Unconjugated(bs-11478R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

SEMA4F Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-11478R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-11478R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-11478R-A555 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-11478R-A647 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, Cy3 Conjugated - bs-11478R-Cy3 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, Cy5 Conjugated - bs-11478R-Cy5 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, Cy5.5 Conjugated - bs-11478R-Cy5.5 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, Cy7 Conjugated - bs-11478R-Cy7 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, FITC Conjugated - bs-11478R-FITC from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, Biotin Conjugated - bs-11478R-Biotin from Bioss

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SEMA4F Polyclonal Antibody, HRP Conjugated - bs-11478R-HRP from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SEMA4F antibody produced in mouse - SAB1412792 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
<B>Immunoblotting</B><BR/>Anti-SEMA4F:<B>Cat. No. SAB1412792</B>: Antibody concentration: 0.1–5 μg/mL. Immunofluorescence band of ~84.7 kDa using SEMA4F transfected 293T lysate.<BR/><B>Lane</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-SEMA4F, (N-terminal) antibody produced in rabbit - SAB2103032 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SEMA4F, antibody produced in rabbit: <B>Cat. No. SAB2103032</B>: Immunoblotting analysis of SEMA4F in transfected 293T cell lysate with SEMA4F anitbody at 1 μg/mL.
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