Gene Symbol | Ppp1r12a |
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Entrez Gene | 17931 |
Alt Symbol | 1200015F06Rik, 5730577I22Rik, AA792106, AV099298, D10Ertd625e, Mypt1 |
Species | Mouse |
Gene Type | protein-coding |
Description | protein phosphatase 1, regulatory (inhibitor) subunit 12A |
Other Description | myosin phosphatase target subunit 1|myosin phosphatase, target subunit 1|myosin phosphatase-targeting subunit 1|protein phosphatase 1 regulatory subunit 12A |
Swissprots | Q99NB6 Q8CBV2 Q05A74 Q9DBR7 |
Accessions | BAB39108 EDL21699 EDL21700 EDL21702 EDL21703 Q9DBR7 AK004785 BAB23563 AK030460 AK035230 BAC28990 AK077739 AK139570 AK162199 BAE36787 AK182043 BC054076 BC125381 AAI25382 BC137630 AAI37631 XM_006513326 XP_006513389 XM_006513327 XP_006513390 XM_006513328 XP_006513391 XM_006513329 XP_006513392 NM_027892 NP_082168 |
Function | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity (By similarity). {ECO:0000250}. |
Subcellular Location | Cytoplasm {ECO:0000250}. Note=Along actomyosin filaments and stress fibers. {ECO:0000250}. |
Tissue Specificity | Expressed in striated and vascular smooth muscle, specificcally in type 2a fibers (at protein level). Expression levels are 20-30% higher in developed males than females (at protein level). {ECO:0000269|PubMed:20634291}. |
Top Pathways | Focal adhesion, Vascular smooth muscle contraction, Oxytocin signaling pathway, cGMP-PKG signaling pathway, cAMP signaling pathway |
MYPT1 CRISPR/Cas9 KO Plasmid (m) - sc-421809 from Santa Cruz Biotechnology
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MYPT1 HDR Plasmid (m) - sc-421809-HDR from Santa Cruz Biotechnology
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MYPT1 Double Nickase Plasmid (m) - sc-421809-NIC from Santa Cruz Biotechnology
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MYPT1 Double Nickase Plasmid (m2) - sc-421809-NIC-2 from Santa Cruz Biotechnology
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MYPT1 CRISPR Activation Plasmid (m) - sc-421809-ACT from Santa Cruz Biotechnology
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MYPT1 CRISPR Activation Plasmid (m2) - sc-421809-ACT-2 from Santa Cruz Biotechnology
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MYPT1 Lentiviral Activation Particles (m) - sc-421809-LAC from Santa Cruz Biotechnology
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MYPT1 Lentiviral Activation Particles (m2) - sc-421809-LAC-2 from Santa Cruz Biotechnology
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MYPT1 siRNA (m) - sc-37241 from Santa Cruz Biotechnology
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MYPT1 shRNA Plasmid (m) - sc-37241-SH from Santa Cruz Biotechnology
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MYPT1 shRNA (m) Lentiviral Particles - sc-37241-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU213881 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN39160 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND39160 from Sigma-Aldrich
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PPP1R12A siRNA (Mouse) - CRM2694 from Cohesion Biosciences
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shRNA set against Mouse PPP1R12A (NM_027892.2) - SHH381012 from Creative biogene
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shRNA set against Mouse Ppp1r12a(NM_027892.2) - SHR133098 from Creative biogene
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PPP1R12A shRNA Plasmid (Mouse) - CHM2694 from Cohesion Biosciences
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PPP1R12A miRNA 3'UTR clone - MiUTR1M-09487 from Creative biogene
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