RAD9

Gene Symbol RAD9
Entrez Gene 851803
Alt Symbol -
Species Yeast
Gene Type protein-coding
Description chromatin-binding protein RAD9
Other Description -
Swissprots P14737 D6VSK0 Q04920
Accessions DAA12060 P14737 NM_001180525 NP_010503
Function Essential for cell cycle arrest at the G2 stage following DNA damage by X-irradiation or inactivation of DNA ligase.
Subcellular Location Nucleus.
Top Pathways Cell cycle - yeast, Meiosis - yeast

Rad9 (yC-20) - sc-6742 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad9 (yC-20): sc-6742. Western blot analysis of yeast recombinant Rad9 fusion protein.

Rad9 (y-300) - sc-50442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Rad9 (yN-19) - sc-6740 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RAD9 pSer1129 antibody - PAB11309 from Acris Antibodies

Prices $580.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RAD9 pSer1260 antibody - PAB11310 from Acris Antibodies

Prices $580.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoblotting of RAD9 (phospho S1260) polyclonal antibody ( Cat # PAB11310 ) to yeast RAD9 at pS1260 was usedat a 1 : 200 dilution incubated overnight at 4°C to detect RAD9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 ( amino acids 991-1309, ~60 kDa ) on a 4-20% Criterion gel for SDS-PAGE. Lane 1, non-phosphorylated wild type yeast RAD9. Lane 2, in vitro phosphorylated wild type yeast RAD9. Lane 3, non-phosphorylated S1129A/S1260A double mutant RAD9. Lane 4, in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of RAD9 was by treatment with ATP and RAD53 kinase. RAD53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110kDa bands ( asterisk ).

RAD9 antibody - PAB11362 from Acris Antibodies

Prices $580.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Affinity purified antibody to yeast RAD9 ( 1125-1139 pan reactive ) was used at a 1:200 dilution incubated 8 h at room temperature to detect RAD9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 ( aa 991-1309 ~60 kDa ) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast RAD9, Lane 2 - in vitro phosphorylated wild type yeast RAD9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant RAD9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of RAD9 was by treatment with ATP and Rad53 kinase.

RAD9 antibody - PAB11363 from Acris Antibodies

Prices $580.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Yeast Rad9 pS1260 (Rabbit) Antibody - GWB-B7A3A0 from Genway Biotech

Prices $500.00
Sizes 100 μg
Host Rabbit

Anti-Yeast Rad9 S1260 (pan reactive) (Rabbit) Antibody - GWB-A355F8 from Genway Biotech

Prices $500.00
Sizes 100 μg
Host Rabbit

Anti-Yeast Rad9 pS1129 Antibody - GWB-8C7440 from Genway Biotech

Prices $500.00
Sizes 100 μg
Host Rabbit

Yeast RAD9 Antibody - GWB-7DC61D from Genway Biotech

Prices $394.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Yeast Rad9 S1129 (pan reactive) Antibody - GWB-29B6C7 from Genway Biotech

Prices $500.00
Sizes 100 μg
Host Rabbit

Anti-RAD9 (aa 1125-1139) polyclonal antibody - DPAB-DC3541 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-RAD9 (aa 1249-1263) polyclonal antibody - DPAB-DC3542 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

RAD9, Polyclonal Antibody - MBS222775 from MyBioSource

Prices $380.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Rad9 phospho S1129 Antibody - 600-401-345 from Rockland Immunochemicals Inc

Prices $319.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Checkpoints are mechanisms that impose delays in the cell cycle in response to DNA damage or defects in DNA replication, to ensure that mitotic transmission is error-free. Failure to delay the cell cycle in the presence of damage converts an easily reparable DNA lesion into one far more deleterious, provoking genomic instability or cell death. This figure shows a summary of our current knowledge about the DNA damage checkpoints in yeast. Genetic analysis of the pathway has allowed classification of its components into Sensors, which detect different sorts of damage, Signal transducers which are signal-integrating kinases, and Targets which carry out the essential functions of suppressing progress through the cell cycle (i.e. inducing repair genes and preventing late origin firing or sister chromatid segregation. Contributed by C. Frei and K. Shimada, laboratory of S. Gasser, U. Geneva.

Rad9 S1260 Antibody - 600-401-467 from Rockland Immunochemicals Inc

Prices $319.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RAD9 Antibody - AHP1294 from AbD Serotec

Prices $270.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

RABBIT ANTI YEAST RAD9 Antibody (50ug) - OASA08924 from Aviva Systems Biology

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Yeast-RAD9-Antibody- - ABD10982 from Abgent, a WuXi AppTec company

Prices $290.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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