KCMF1

Gene Symbol	KCMF1
Entrez Gene	56888
Alt Symbol	DEBT91, FIGC, PCMF, ZZZ1
Species	Human
Gene Type	protein-coding
Description	potassium channel modulatory factor 1
Other Description	E3 ubiquitin-protein ligase KCMF1\|FGF-induced in gastric cancer\|FGF-induced ubiquitin-protein ligase in gastric cancers\|ZZ-type zinc finger-containing protein 1\|differentially expressed in branching tubulogenesis 91\|zinc finger, ZZ domain containing 1
Swissprots	Q9UFE8 Q4ZG04 Q53SC7 Q9P0J7 Q9H8P5 Q9BWK2
Accessions	AAX88897 AAY24192 EAW99544 EAW99545 EAW99546 Q9P0J7 AB083199 BAC43745 AF131740 AF155652 AAF67009 AK023403 BAB14563 AK311099 AK314761 BAG37299 AL122115 CAB59274 AW466865 BC000178 AAH00178 BU844982 CT004343 DQ894079 ABM85005 XM_006712052 XP_006712115 XM_011532990 XP_011531292 NM_020122 NP_064507
Function	Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination. {ECO:0000269\|PubMed:15581609}.
Tissue Specificity	Spleen, small intestine, ovary, peripheral blood, lung, kidney and pancreas. Expressed at low levels in the thymus, prostate, testis, colon, heart, brain, placenta and liver. {ECO:0000269\|PubMed:15581609}.

KCMF1 (A-3) - sc-390051 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

KCMF1 (A-3): sc-390051. Western blot analysis of KCMF1 expression in WI 38 (A) and SW480 (B) whole cell lysates.

KCMF1 (E-12) - sc-161087 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

KCMF1 (E-12): sc-161087. Western blot analysis of KCMF1 expression in human PBL (A) and SW480 (B) whole cell lysates.

KCMF1 (H-129) - sc-382290 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Anti-PCMF antibody - ab80287 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PCMF antibody (ab80287) at 1 µg/ml + transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PCMF antibody - C-terminal - ab170382 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PCMF antibody - C-terminal (ab170382) at 1/100 dilutionLane 1 : HepG2 cell lysateLane 2 : Mouse NIH3T3 cell lysateLysates/proteins at 35 µg per lane.

Anti-KCMF1 Antibody (aa280-308, AP) - LS-C236699 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (clone 2483C5a) - LS-C184807 from LifeSpan Bioscience

Prices	$335.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	2483C5a

Anti-KCMF1 Antibody (aa280-308, APC) - LS-C236700 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (aa280-308, Biotin) - LS-C236701 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (aa280-308, FITC) - LS-C236702 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (aa280-308, PE) - LS-C236704 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (aa280-308, Azide-free, HRP) - LS-C249295 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (aa287-316) - LS-C322365 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 Antibody (clone 2483C5a) - LS-C342814 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2483C5a

Anti-KCMF1 Antibody (aa280-308) - LS-C165407 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

KCMF1 antibody western blot of HepG2,mouse NIH-3T3 cell line lysates (35 ug/lane). The KCMF1 antibody detected the KCMF1 protein (arrow).

Anti-KCMF1 Antibody (aa71-120) - LS-C80031 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-KCMF1 Antibody (C-Terminus) - LS-C80259 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-KCMF1 Antibody (aa50-100) - LS-C290715 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human KCMF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KCMF1 antibody used for IP at 6 ug/mg lysate. KCMF1 was also immunoprecipitated by rabbit anti-KCMF1 antibody BL14690. For blotting immunoprecipitated KCMF1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

KCMF1 Antibody - NBP1-84284 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: KCMF1 Antibody [NBP1-84284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412662)

KCMF1 Antibody - NBP1-84283 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: KCMF1 Antibody [NBP1-84283] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Anti-KCMF1 (ab1) antibody produced in rabbit - SAB2101199 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-KCMF1 (ab1): <B>Cat. No.SAB2101199</B>: KCMF1 in Transfected 293T cell lysate with KCMF1 antibody at 1.0 μg/mL.

Anti-KCMF1 antibody produced in rabbit - HPA030383 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-KCMF1: <B>Cat. No. HPA030383</B>: Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine and endocrine cells.

Anti-KCMF1 (246-260) antibody produced in rabbit - SAB1105348 from Sigma-Aldrich

Prices	$279.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 (146-160) antibody produced in rabbit - SAB1105347 from Sigma-Aldrich

Prices	$279.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KCMF1 antibody produced in rabbit - HPA030384 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-KCMF1: <B>Cat. No. HPA030384</B>: Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in glandular cells.

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