Pnpla2

Gene Symbol Pnpla2
Entrez Gene 66853
Alt Symbol 0610039C21Rik, 1110001C14Rik, Atgl, TTS-2.2
Species Mouse
Gene Type protein-coding
Description patatin-like phospholipase domain containing 2
Other Description adipose triglyceride lipase|desnutrin|patatin-like phospholipase domain-containing protein 2|transport-secretion protein|triglyceride hydrolase
Swissprots Q05BJ0 O89080 Q8BJ56 Q3UD97 Q643S0 Q9DCF6 Q6P234 Q9D1Q9
Accessions EDL18088 EDL18089 EDL18090 EDL18091 EDL18092 EDL18093 Q8BJ56 AK002826 BAB22387 AK003207 BAB22643 AK015984 AK031609 BAC27476 AK076464 AK085926 AK150184 BAE29364 AK183970 AK185834 AY510273 AAS48458 AY731699 AAU33824 AY894805 AAW81963 BB574719 BC019188 AAH19188 BC026807 BC034190 BC044737 BC044781 AAH44781 BC054825 BC064747 AAH64747 CF725975 U89431 AAC36536 NM_001163689 NP_001157161 NM_025802 NP_080078 NR_028142
Function Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. {ECO:0000269|PubMed:15337759, ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:16150821, ECO:0000269|PubMed:16675698, ECO:0000269|PubMed:16679289, ECO:0000269|PubMed:17074755}.
Subcellular Location Lipid droplet. Cell membrane; Single-pass type II membrane protein.
Tissue Specificity Expressed at high levels in white and brown adipose tissue, and to a lesser degree in testis and cardiac muscle. Barely detected in liver, spleen, thymus, kidney, skeletal muscle, and brain. Among the white adipose depots, gonadal fat showed the highest level of expression compared with inguinal and renal white adipose tissues. {ECO:0000269|PubMed:15337759, ECO:0000269|PubMed:15550674, ECO:0000269|PubMed:16150821, ECO:0000269|PubMed:16705060}.
Top Pathways Glycerolipid metabolism

ATGL (E-12) - sc-50220 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Adipose Triglyceride Lipase (phospho S406) antibody - ab135093 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/mlLane 1 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue LysateLane 2 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue LysateLane 3 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue Lysate with Mouse Adipose Triglyceride Lipase (phospho S406) peptide (ab174058) at 1 µg/mlLane 4 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate with Mouse Adipose Triglyceride Lipase (phospho S406) peptide (ab174058) at 1 µg/mlLane 5 : Mouse (Male) WAT from Gonard (High Fat Diet) Tissue Lysate with Mouse Adipose Triglyceride Lipase peptide (ab174059) at 1 µg/mlLane 6 : Mouse WAT (White Adipose Tissue) (Fasted) Tissue Lysate with Mouse Adipose Triglyceride Lipase peptide (ab174059) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

PEDF R/PNPLA2/ATGL Antibody (615728) - MAB5365 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Rat
Clonality Monoclonal
Clone 615728
Western blot shows lysates of mouse adipose tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse PEDF R/PNPLA2 Monoclonal Antibody (Catalog # MAB5365) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PEDF R/PNPLA2 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 10.

PEDF R/PNPLA2/ATGL Antibody - NB100-57854 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: PEDF R/PNPLA2/ATGL Antibody [NB100-57854] - Staining of Human Adipose lysate.

PNPLA2 / ATGL antibody - PAB5923 from Acris Antibodies

Prices $420.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ATGL Antibody - 45-306 from ProSci

Prices $350.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot (0.5μg/ml) staining of Human Adipose lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ATGL antibody - STJ71118 from St John's Laboratory Ltd

Host Goat
Clonality Polyclonal

Anti-PNPLA2 (aa 68-79) polyclonal antibody - DPABH-28139 from Creative Diagnostics

Host Goat
Clonality Polyclonal

PNPLA2 Antibody - TGB-971 from Reagent Proteins

Prices $348.00, $675.00
Sizes 20.00 µg, 100.00 µg
Host Mouse
Clonality Monoclonal

ATGL - RA25015 from Neuromics

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Image: Detection of ATGL in human adipose tissue lysate.

ATGL Monoclonal Antibody (K.751.7) - MA5-14990 from Thermo Fisher Scientific

Prices $275.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone K.751.7
Immunofluorescent analysis of ATGL in 3T3-L1 cells using an ATGL monoclonal antibody (Product # MA5-14990) (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.

ATGL - ABA-576-06100 from Nordic BioSite

Host Rabbit

PNPLA2, Polyclonal Antibody - MBS418316 from MyBioSource

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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