AAK1

Gene Symbol AAK1
Entrez Gene 22848
Alt Symbol -
Species Human
Gene Type protein-coding
Description AP2 associated kinase 1
Other Description AP2-associated protein kinase 1|adaptor-associated kinase 1
Swissprots Q4ZFZ3 Q2M2I8 Q9UPV4 Q53RX6
Accessions AAX88861 AAY14931 EAW99847 EAW99848 Q2M2I8 AB028971 BAA83000 AF090100 AF090101 AK055622 AK056043 AK074292 AK098797 AK124872 AK127187 BAC86877 AK310951 AW451954 AW969393 BC002695 AAH02695 BC073953 BC090950 BC104842 AAI04843 BC111965 AAI11966 BC132937 BC132939 BC143710 AAI43711 BX640963 BX641060 CA436375 DA121999 DB285703 NM_014911 NP_055726
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. {ECO:0000269|PubMed:12952931, ECO:0000269|PubMed:17494869, ECO:0000269|PubMed:18657069, ECO:0000269|PubMed:21464124}.
Subcellular Location Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Membrane, clathrin-coated pit {ECO:0000250}. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity). {ECO:0000250}.
Tissue Specificity Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain. {ECO:0000269|PubMed:17494869}.

AAK1 (A-14) - sc-167025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AAK1 (C-15) - sc-130105 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

AAK1 (H-140) - sc-134662 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

AAK1 (LW-M11) - sc-134242 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
AAK1 (LW-M11): sc-134242. Western blot analysis of AAK1 expression in truncated human AAK1 transfected (A) and non-transfected (B) 293T whole cell lysates.

Anti-AAK1 antibody - ab77082 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-AAK1 antibody (ab77082) at 1 µg/mlLane 2 : Anti-AAK1 antibody (ab77082) at 2 µg/mlLane 1 : A-20 lysate Lane 2 : A-20 lysate Lysates/proteins at 15 µg per lane.

Anti-AAK1 antibody - ab59740 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AAK1 antibody (ab59740) at 0.5 µg/mlLane 1 : Mouse tissue lysateLane 2 : Rat kidney tissue lysate

Anti-AAK1 antibody - ab66005 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-AAK1 antibody (ab66005) at 1/500 dilutionLane 1 : Extracts from COS7 cellsLane 2 : Extracts from COS7 cells, plus 5µg immunizing peptideLysates/proteins at 5 µg per lane.

Anti-AAK1 antibody - ab168518 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-AAK1 antibody (ab168518) at 1/500 dilutionLane 1 : AAK1 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysate

Anti-AAK1 antibody [EPR5126(2)] - ab134971 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5126(2)
All lanes : Anti-AAK1 antibody [EPR5126(2)] (ab134971) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-AAK1 Antibody (C-Terminus) IHC-plus™ - LS-B3833 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-AAK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3833 concentration 5 ug/ml.

Anti-AAK1 Antibody (N-Terminus) IHC-plus™ - LS-B3843 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-AAK1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3843 concentration 5 ug/ml.

Anti-AAK1 Antibody - LS-C48729 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of AAK1 antibody LS-C48729.

Anti-AAK1 Antibody (aa677-691) - LS-C112877 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AAK1 antibody (1 ug/ml) staining of Human Amygdala lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AAK1 Antibody (aa593-642) - LS-C145232 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AAK1 antibody LS-C145232 Western blot of Mouse Thymus lysate. Antibody concentration 1 ug/ml.

Anti-AAK1 Antibody (aa790-819) - LS-C97469 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-AAK1 Antibody in HepG2 cell line lysates (35 ug/lane). AAK1 (arrow) was detected using the purified antibody.

Anti-AAK1 Antibody (aa271-320) - LS-C119088 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from COS7 cells, using AAK1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-AAK1 Antibody (C-Terminus) - LS-C135333 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AAK1 antibody LS-C135333 Western blot of 721_B cell lysate.

Anti-AAK1 Antibody (aa704-822, clone 12K1) - LS-C182750 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 12K1

Anti-AAK1 Antibody (aa279-306, Biotin) - LS-C232290 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AAK1 Antibody (aa279-306, APC) - LS-C232288 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AAK1 Antibody (aa279-306, AP) - LS-C232289 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AAK1 Antibody (aa279-306, FITC) - LS-C232291 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AAK1 Antibody (aa279-306, PE) - LS-C232294 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AAK1 Antibody (aa310-360) - LS-C288897 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AAK1 Antibody (aa186-216) - LS-C155831 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of AAK1 Antibody in Ramos cell line lysates (35 ug/lane). AAK1 (arrow) was detected using the purified antibody.
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