HECTD1

Gene Symbol HECTD1
Entrez Gene 25831
Alt Symbol EULIR
Species Human
Gene Type protein-coding
Description HECT domain containing E3 ubiquitin protein ligase 1
Other Description E3 ligase for inhibin receptor|E3 ubiquitin-protein ligase HECTD1|HECT domain-containing protein 1
Swissprots Q6P445 Q96F34 Q86VJ1 D3DS86 Q9ULT8 Q9UFZ7
Accessions EAW65950 EAW65951 EAW65952 Q9ULT8 AB032957 BAA86445 AK024847 AK095634 AK125788 BAG54248 AL110222 CAB53681 AY254380 AAP13073 AY739714 AAW65983 BC006237 AAH06237 BC011658 AAH11658 BC016947 BC063686 AAH63686 BC072441 BC083494 AAH83494 BC172391 AAI72391 BM977042 DA440215 DA509926 DA818448 XM_005267502 XP_005267559 XM_005267503 XP_005267560 XM_011536619 XP_011534921 XM_011536620 XP_011534922 XM_011536621 XP_011534923 XM_011536622 XP_011534924 XR_943414 NM_015382 NP_056197
Function Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. May be required for development of the head mesenchyme and neural tube closure (By similarity). {ECO:0000250}.

EULIR (H-300) - sc-134976 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

EULIR (N-20) - sc-67563 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EULIR (D-15) - sc-67562 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EULIR (C-14) - sc-67561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EULIR (C-14): sc-67561. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.

Anti-HECTD1 antibody - ab101992 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab101992 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101992 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HECTD1 antibody - ab57052 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57052 HECTD1 antibody at 1ug/ml.

Anti-HECTD1 antibody - ab101993 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101993 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes

Anti-HECTD1 Antibody (clone 1C7) - LS-C139213 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C7

Anti-HECTD1 Antibody (aa2100-2150) - LS-C289618 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HECTD1 Antibody (aa1-50) IHC-plus™ - LS-B11751 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

HECTD1 Antibody (1E10) - H00025831-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E10

HECTD1 Antibody - NBP1-49926 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: HECTD1 Antibody [NBP1-49926] - Detection of Human HECTD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HECTD1 antibody used for IP at 3 mcg/mg lysate. HECTD1 was also immunoprecipitated by rabbit anti-HECTD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated HECTD1 was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

HECTD1 Antibody - NBP1-49925 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: HECTD1 Antibody [NBP1-49925] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HECTD1 antibody NBP1-49925 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HECTD1 was also immunoprecipitated by rabbit anti-HECTD1 antibody NBP1-49926, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

HECTD1 Antibody - NBP1-82926 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: HECTD1 Antibody [NBP1-82926] - Staining of human cerebral cortex shows moderate positivity in neurons.

HECTD1 Antibody (1C7) - H00025831-M08 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C7

HECTD1 Polyclonal Antibody - bs-15446R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-HECTD1 antibody produced in mouse - WH0025831M3 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E10
<B>Immunoblotting</B><BR/>Monoclonal Anti-HECTD1, <B>Cat. No. WH0025831M3</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37.99 kDa using immunogen protein lysate.

Anti-HECTD1 antibody produced in rabbit - HPA002929 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-HECTD1: <B>Cat. No. HPA002929</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cerebral cortex shows moderate positivity in neurons.

HECTD1-Antibody-monoclonal-M03 - AT2345a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1E11
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.62 KDa) .

HECTD1 antibody - orb67186 from Biorbyt

Prices $425.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Western blot analysis of total cell extracts from human Hela using HECTD1 antibody

HECTD1 antibody - 70R-17716 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
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