Rhox5

Gene Symbol Rhox5
Entrez Gene 18617
Alt Symbol AA409564, Pem
Species Mouse
Gene Type protein-coding
Description reproductive homeobox 5
Other Description homeobox protein Pem|homeobox protein Rhox5|placenta and embryonic expression protein|placentae and embryos oncofetal|reproductive homeobox on X chromosome, 5|reproductive homeobox on chromosome X 5
Swissprots P52651
Accessions AAA96048 AAL35322 EDL28998 P52651 AK146379 BAE27127 AK146393 BAE27136 AK146449 BAE27181 BC056204 AAH56204 DQ058642 AAY58253 M32484 AAC42025 NM_008818 NP_032844
Function Possible transcription factor which is developmentally regulated.
Subcellular Location Nucleus {ECO:0000305}.

Pem (M-15) - sc-21650 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pem (M-15): sc-21650. Western blot analysis of Pem expression in I-11.15 (A) and C3H/10T1/2 (B) whole cell lysates.

Anti-Rhox5 / PEM antibody - ab31922 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab31922 stained NIH 3T3 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31922 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-RHOX5 Antibody (aa19-32) - LS-C47427 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-RHOX5 (aa 100-200) polyclonal antibody - CABT-BL3153 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal
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