Gene Symbol | Rad50 |
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Entrez Gene | 19360 |
Alt Symbol | Mrell, Rad50l |
Species | Mouse |
Gene Type | protein-coding |
Description | RAD50 homolog (S. cerevisiae) |
Other Description | DNA repair protein RAD50|mRad50 |
Swissprots | P70388 Q6PEB0 Q8C2T7 Q9CU59 |
Accessions | EDL33574 P70388 AK018001 BAB31030 AK037641 AK087982 BAC40074 AK132949 BAE21438 AK144155 BAE25735 BC002033 AAH02033 BC058180 AAH58180 BC168398 AAI68398 U66887 AAC52894 NM_009012 NP_033038 |
Function | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity). {ECO:0000250}. |
Subcellular Location | Nucleus {ECO:0000250}. Chromosome, telomere {ECO:0000250}. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. {ECO:0000250}. |
Tissue Specificity | In adult, it is expressed at very low level in most tissues, except in heart, lung and aorta. Expressed at high level in testis. {ECO:0000269|PubMed:8910585}. |
Top Pathways | Homologous recombination, Non-homologous end-joining |
Rad50 CRISPR/Cas9 KO Plasmid (m) - sc-422580 from Santa Cruz Biotechnology
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Rad50 HDR Plasmid (m) - sc-422580-HDR from Santa Cruz Biotechnology
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Rad50 Double Nickase Plasmid (m) - sc-422580-NIC from Santa Cruz Biotechnology
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Rad50 Double Nickase Plasmid (m2) - sc-422580-NIC-2 from Santa Cruz Biotechnology
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Rad50 CRISPR Activation Plasmid (m) - sc-422580-ACT from Santa Cruz Biotechnology
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Rad50 CRISPR Activation Plasmid (m2) - sc-422580-ACT-2 from Santa Cruz Biotechnology
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Rad50 Lentiviral Activation Particles (m) - sc-422580-LAC from Santa Cruz Biotechnology
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Rad50 Lentiviral Activation Particles (m2) - sc-422580-LAC-2 from Santa Cruz Biotechnology
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Rad50 siRNA (m) - sc-37398 from Santa Cruz Biotechnology
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Rad50 shRNA Plasmid (m) - sc-37398-SH from Santa Cruz Biotechnology
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Rad50 shRNA (m) Lentiviral Particles - sc-37398-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU045741 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN39829 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND39829 from Sigma-Aldrich
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shRNA set against Mouse RAD50 (NM_009012.2) - SHH388988 from Creative biogene
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shRNA set against Mouse Rad50(NM_009012.2) - SHR162022 from Creative biogene
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RAD50 miRNA 3'UTR clone - MiUTR1M-09885 from Creative biogene
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