Gene Symbol | NEMF |
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Entrez Gene | 9147 |
Alt Symbol | NY-CO-1, SDCCAG1 |
Species | Human |
Gene Type | protein-coding |
Description | nuclear export mediator factor |
Other Description | antigen NY-CO-1|nuclear export mediator factor NEMF|serologically defined colon cancer antigen 1 |
Swissprots | Q9NWG1 Q8WW70 B4E3F3 B4DHA9 A0JLQ3 Q32Q66 B4DDL3 B3KSK1 O60524 |
Accessions | CCQ43274 CCQ43275 EAW65741 EAW65742 EAW65743 EAW65744 EAW65745 EAW65746 EAW65747 O60524 AF039687 AAC18036 AK000913 AK093783 BAG52763 AK293236 BAG56774 AK295010 BAG58070 AK304695 BAG65465 AK308537 AK314281 BC006001 AAH06001 BC020794 AAH20794 BC031007 BC041351 BC056687 AAH56687 BC064364 AAH64364 BC107764 AAI07765 BC146281 AAI46282 BC148732 AAI48733 BM477286 BM835709 BX640804 CAE45886 BX640807 CAE45889 BX648598 BX648753 CB112336 DA899175 DC382921 XM_005268200 XP_005268257 XM_005268201 XP_005268258 XM_011537316 XP_011535618 XM_011537317 XP_011535619 XM_011537318 XP_011535620 XR_943556 XR_943557 NM_001301732 NP_001288661 NM_004713 NP_004704 |
Function | Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875). {ECO:0000269|PubMed:16103875, ECO:0000269|PubMed:25578875}. |
Subcellular Location | Nucleus {ECO:0000305}. |
Tissue Specificity | Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis. {ECO:0000269|PubMed:16103875}. |
SDCCAG1 CRISPR/Cas9 KO Plasmid (h) - sc-411248 from Santa Cruz Biotechnology
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SDCCAG1 HDR Plasmid (h) - sc-411248-HDR from Santa Cruz Biotechnology
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SDCCAG1 Double Nickase Plasmid (h) - sc-411248-NIC from Santa Cruz Biotechnology
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SDCCAG1 Double Nickase Plasmid (h2) - sc-411248-NIC-2 from Santa Cruz Biotechnology
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SDCCAG1 CRISPR Activation Plasmid (h) - sc-411248-ACT from Santa Cruz Biotechnology
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SDCCAG1 CRISPR Activation Plasmid (h2) - sc-411248-ACT-2 from Santa Cruz Biotechnology
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SDCCAG1 Lentiviral Activation Particles (h) - sc-411248-LAC from Santa Cruz Biotechnology
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SDCCAG1 Lentiviral Activation Particles (h2) - sc-411248-LAC-2 from Santa Cruz Biotechnology
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SDCCAG1 siRNA (h) - sc-92343 from Santa Cruz Biotechnology
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SDCCAG1 shRNA Plasmid (h) - sc-92343-SH from Santa Cruz Biotechnology
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SDCCAG1 shRNA (h) Lentiviral Particles - sc-92343-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU057811 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18991 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18991 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06429 from Sigma-Aldrich
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NEMF siRNA (Human) - CRH6053 from Cohesion Biosciences
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shRNA set against Human NEMF (NM_004713.3) - SHH351358 from Creative biogene
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shRNA set against Human SDCCAG1(NM_004713.3) - SHR191712 from Creative biogene
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Human NEMF siRNA - orb266555 from Biorbyt
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NEMF shRNA Plasmid (Human) - CHH6053 from Cohesion Biosciences
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NEMF miRNA 3'UTR clone - MiUTR1H-09209 from Creative biogene
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