MBD1

Gene Symbol MBD1
Entrez Gene 4152
Alt Symbol CXXC3, PCM1, RFT
Species Human
Gene Type protein-coding
Description methyl-CpG binding domain protein 1
Other Description CXXC-type zinc finger protein 3|methyl-CpG binding domain protein 1 isoform PCM1|methyl-CpG-binding domain protein 1|protein containing methyl-CpG-binding domain 1|the regulator of fibroblast growth factor 2 (FGF-2) transcription
Swissprots K7ESN0 E9PEC5 Q9UNZ8 A4UTZ0 O95241 K7ELI2 Q9UIS9 Q9UNZ6 Q8N4W4 K7EQZ4 Q7Z7B5 B4DXJ5 O15248 Q9UNZ7 Q9UNZ9
Accessions AAD50371 EAW62962 EAW62963 EAW62964 EAW62965 EAW62966 EAW62967 Q9UIS9 AB209765 BAD93002 AF072241 AAC68870 AF078830 AAD51442 AF078831 AAD51443 AF078832 AAD51444 AF078833 AAD51445 AJ564845 CAD92308 AK225889 AK225948 AK291519 BAF84208 AK295401 BAG58353 AK300759 BAG62425 AK302004 BAG63407 BC033242 AAH33242 BC047353 BP384783 CD723822 DB458244 DC403953 EF488685 ABP02056 Y10746 CAA71735 XM_005258271 XP_005258328 XM_006722456 XP_006722519 XM_011525987 XP_011524289 XM_011525988 XP_011524290 XM_011525989 XP_011524291 XM_011525990 XP_011524292 XM_011525991 XP_011524293 XM_011525992 XP_011524294 XM_011525993 XP_011524295 XM_011525994 XP_011524296 XM_011525995 XP_011524297 XM_011525996 XP_011524298 XM_011525997 XP_011524299 XM_011525998 XP_011524300 XM_011525999 XP_011524301 XM_011526000 XP_011524302 XM_011526001 XP_011524303 XM_011526002 XP_011524304 XM_011526003 XP_011524305 XM_011526004 XP_011524306 XM_011526005 XP_011524307 XM_011526006 XP_011524308 XM_011526007 XP_011524309 NM_001204136 NP_001191065 NM_001204137 NP_001191066 NM_001204138 NP_001191067 NM_001204139 NP_001191068 NM_001204140 NP_001191069 NM_001204141 NP_001191070 NM_001204142 NP_001191071 NM_001204143 NP_001191072 NM_001204151 NP_001191080 NM_002384 NP_002375 NM_015844 NP_056669 NM_015845 NP_056670 NM_015846 NP_056671 NM_015847 NP_056723
Function Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. {ECO:0000269|PubMed:10454587, ECO:0000269|PubMed:10648624, ECO:0000269|PubMed:12665582, ECO:0000269|PubMed:12697822, ECO:0000269|PubMed:12711603, ECO:0000269|PubMed:14555760, ECO:0000269|PubMed:14610093, ECO:0000269|PubMed:15327775, ECO:0000269|PubMed:9207790, ECO:0000269|PubMed:9774669}.
Subcellular Location Nucleus {ECO:0000250}. Nucleus matrix {ECO:0000250}. Nucleus speckle. Chromosome. Note=Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. {ECO:0000250}.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:9207790}.

MBD1 (A-5) - sc-55473 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MBD1 (A-5): sc-55473. Western blot analysis of MBD1 expression in A-431 (A) and MM-142 (B) nuclear extracts.

MBD1 (B-5) - sc-25261 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MBD1 (B-5): sc-25261. Western blot analysis of MBD1 expression in A-431 (A), MM-142 (B) and KNRK (C) nuclear extracts.

MBD1 (G-18) - sc-9395 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MBD1 (G-18): sc-9395. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear and cytoplasmic staining of glandular cells.

MBD1 (M-254) - sc-10751 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MBD1 (M-254): sc-10751.Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.

MBD1 (N-15) - sc-9394 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MBD1 (N-15): sc-9394. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing nuclear and cytoplasmic staining of glandular cells.

MBD1 (100B272.1) - sc-52901 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MBD1 (100B272.1): sc-52901. Western blot analysis of MBD1 expression in Jurkat whole cell lysate (A) and Hep G2 (B), MEG-01 (C) and U-937 (D) nuclear extracts.

Anti-MBD1 antibody - ab124519 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MBD1 antibody (ab124519) at 4 µg/ml + Jurkat cell lysate

Anti-MBD1 antibody - ab89534 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-MBD1 antibody (ab89534) at 5 µg/ml + Partial tagged recombinant Human MBD1 protein at 0.2 µg

Anti-MBD1 antibody [100B272.1.] - ChIP Grade - ab45028 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 100B272.1.
Lane 1 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 2 µg/mlLane 2 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 1 µg/mlLane 1 : HeLa cell lysate Lane 2 : HeLa cell lysate

Anti-MBD1 antibody [EPR3564] - ab108510 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3564
All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.

Anti-MBD1 antibody - ChIP Grade - ab2846 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MBD1 Antibody (aa366-381) - LS-C313166 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of MBD1 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human MBD1, 38.2KD. (162aa tag+ R283-A463). All lanes: Anti-MBD1 at 0.5ug/ml. Lane 1: Recombinant Human MBD1 Protein 10ng. Lane 2: Recombinant Human MBD1 Protein 5ng. Predicted bind size: 67KD. Observed bind size: 67KD.

Anti-MBD1 Antibody (clone 2C7) - LS-C133910 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C7
Immunoperoxidase of monoclonal antibody to MBD1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]

Anti-MBD1 Antibody (Internal, clone EPR3564) - LS-C137713 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3564
IHC of paraffin-embedded human tonsils using anti-MBD1 antibody.

Anti-MBD1 Antibody (C-Terminus) IHC-plus™ - LS-B1545 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MBD1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1545 concentration 10 ug/ml.

Anti-MBD1 Antibody - LS-C72245 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MBD1 Antibody - LS-C153186 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MBD1 Antibody (aa594) - LS-C190224 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MBD1 Antibody - LS-C308904 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MBD1 Antibody (aa98-113) - LS-C743 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of MBD1 in HeLa cell lysate with anti-MBD1 pcAb. A protein band with an approximate molecular weight of 55 kD was detected.

Anti-MBD1 Antibody (C-Terminus) - LS-C31263 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: Jurkat Cell Lysate

Anti-MBD1 Antibody - LS-C310955 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MBD1 Antibody (N-Terminus) - LS-C347701 from LifeSpan Bioscience

Prices $405.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-MBD1 Antibody (C-Terminus) - LS-C145754 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
MBD1 antibody LS-C145754 Western blot of 293T Cell lysate. Antibody concentration 1 ug/ml.

Anti-MBD1 Antibody (clone 100B272.1.) - LS-C563 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 100B272.1.
Western blot of MBD1 in HeLa cell lysate using antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).
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