SERPINH1

Gene Symbol SERPINH1
Entrez Gene 871
Alt Symbol AsTP3, CBP1, CBP2, HSP47, OI10, PIG14, PPROM, RA-A47, SERPINH2, gp46
Species Human
Gene Type protein-coding
Description serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Other Description 47 kDa heat shock protein|arsenic-transactivated protein 3|cell proliferation-inducing gene 14 protein|colligin-1|colligin-2|rheumatoid arthritis antigen A-47|rheumatoid arthritis-related antigen RA-A47|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)|serpin H1
Swissprots Q9NP88 Q8IY96 P29043 B3KVJ3 Q5XPB4 P50454 Q6NSJ6
Accessions BAA96790 BAA96791 CDL93515 EAW74974 EAW74975 EAW74976 EAW74977 EAW74978 EAW74979 P50454 AB044778 BAA96788 AB044779 BAA96789 AI926019 AK075504 AK094047 AK122936 BAG53805 AK130870 AK131503 AK290124 BAF82813 AK297811 BAG60149 AK309905 AY264853 AAP93914 AY744367 AAU95378 BC014623 AAH14623 BC036298 AAH36298 BC070087 AAH70087 BQ016136 BT007094 AAP35758 CB123399 D83174 BAA11829 DC398178 X61598 CAA43795 XM_006718729 XP_006718792 XM_011545326 XP_011543628 XM_011545327 XP_011543629 NM_001207014 NP_001193943 NM_001235 NP_001226
Function Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Subcellular Location Endoplasmic reticulum lumen.

HSP 47 (E-1) - sc-13150 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSP 47 (E-1): sc-13150. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.

HSP 47 (E-4) - sc-398579 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSP 47 (E-4): sc-398579. Western blot analysis of HSP 47 expression in L8 (A) and NIH/3T3 (B) whole cell lysates.

HSP 47 (G-12) - sc-5293 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of HSP 47 expression in HeLa (A), CCD-1064Sk (B) and L8 (C) whole cell lysates. Antibodies tested include HSP 47 (G-12): sc-5293 (A,B) and HSP 47 (H-300): sc-8352 (C).

HSP 47 (H-300) - sc-8352 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HSP 47 (H-300): sc-8352. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human fetal kidney showing cytoplasmic staining.

HSP 47 (N-17) - sc-7621 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSP 47 (N-17): sc-7621. Western blot analysis of HSP 47 expression in L8 whole cell lysate.

Anti-Hsp47 antibody - ab54874 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HepG2 cells stained with ab54874 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54874, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Hsp47 antibody - ab88115 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Hsp47 antibody (ab88115) at 1 µg/ml + Human pancreas lysate at 25 µgSecondaryGoat anti-Mouse IgG at 1/5000 dilution

Anti-Hsp47 antibody [EPR4217] - ab109117 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4217
ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

Anti-Hsp47 antibody [EPR4217] (Alexa Fluor® 488) - ab192841 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4217
ab192841 staining Hsp47 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192841 at a working dilution of 1 in 50 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor® 594 Goat anti-Mouse secondary (ab150120) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 4% formaldehyde (10 min) fixed A431 cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Hsp47 antibody [EPR4217] (Alexa Fluor® 647) - ab192844 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4217
ab192844 staining Hsp47 alpha in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192844 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 488 Goat anti-Mouse secondary (ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.This product also gave a positive signal in 4% formaldehyde (10min) fixed HeLa cells under the same testing conditions.

Anti-Hsp47 antibody [EPR4217] (HRP) - ab193242 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4217
IHC image of Hsp47 staining in a section of formalin-fixed paraffin-embedded normal human placenta tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193242 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-SERPINH1 / HSP47 Antibody (C-Terminus) - LS-C31759 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (aa406-417) - LS-C15723 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (Internal, clone 134CT3.1.1, AP) - LS-C232243 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 134CT3.1.1

Anti-SERPINH1 / HSP47 Antibody (Internal, clone 134CT3.1.1, Biotin) - LS-C232245 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 134CT3.1.1

Anti-SERPINH1 / HSP47 Antibody (Internal, clone 134CT3.1.1, FITC) - LS-C232246 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 134CT3.1.1

Anti-SERPINH1 / HSP47 Antibody (Internal, clone 134CT3.1.1, PE) - LS-C232248 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 134CT3.1.1

Anti-SERPINH1 / HSP47 Antibody (C-Terminus, AP) - LS-C245771 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (C-Terminus, APC) - LS-C245772 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (C-Terminus, PE) - LS-C245774 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (C-Terminus, FITC) - LS-C245773 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (C-Terminus, Azide-free, HRP) - LS-C247852 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SERPINH1 / HSP47 Antibody (clone 1C4-1A6) - LS-C309123 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C4-1A6

Anti-SERPINH1 / HSP47 Antibody (Internal, clone 134CT3.1.1) - LS-C319204 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 134CT3.1.1

Anti-SERPINH1 / HSP47 Antibody (Internal, clone 134CT3.1.1, Azide-free, HRP) - LS-C249341 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 134CT3.1.1
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