NSL1

Gene Symbol	NSL1
Entrez Gene	25936
Alt Symbol	C1orf48, DC8, MIS14
Species	Human
Gene Type	protein-coding
Description	NSL1, MIS12 kinetochore complex component
Other Description	NSL1, MIND kinetochore complex component, homolog\|kinetochore-associated protein NSL1 homolog
Swissprots	Q96IY1 E7ETD5 Q5SY75 Q9Y415 Q9NRN8 Q9H2M5
Accessions	EAW93379 EAW93380 Q96IY1 AA806390 AF201941 AAF86877 AF255793 AAG44640 AI703127 AK092359 AK124596 AK222996 BAD96716 AK223260 BAD96980 AK303250 BAG64333 AK313730 BAG36472 AL050084 CAB43260 BC007067 AAH07067 BC036048 BE669670 BQ023978 BU570983 CK003923 DB045932 DQ892899 ABM83825 DQ896149 ABM87148 NM_001042549 NP_001036014 NM_001297736 NP_001284665 NM_001297737 NP_001284666 NM_001297739 NP_001284668 NM_015471 NP_056286
Function	Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. {ECO:0000269\|PubMed:16585270}.
Subcellular Location	Nucleus. Chromosome, centromere, kinetochore. Note=Associated with the kinetochore.

NSL1 (S-14) - sc-104465 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NSL1 (E-12) - sc-104464 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-NSL1 antibody - ab96728 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSL1 antibody (ab96728) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-NSL1 antibody - ab70835 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NSL1 with ab70835 at 1/1000 (1µg/ml). Detection: DAB.

Anti-NSL1 antibody - ab70836 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1: ab70835 used for IP at 3 µg/mg lysate. Lane 2: immunoprecipitation with an other antibody Lane 3: ab70836 used for IP at 3 µg/mg lysate Lane 4: Control IgG IP For blotting ab70836 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-NSL1 antibody - ab92532 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab92532 at 1/250 dilution staining NSL1 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-NSL1 Antibody (C-Terminus) - LS-C287652 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSL1 Antibody (aa192-241) - LS-C146220 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

NSL1 antibody LS-C146220 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.

Anti-NSL1 Antibody (aa150-200) - LS-C287651 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human DC8 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DC8 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-NSL1 Antibody (aa150-200) - LS-C286694 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-NSL1 Antibody - LS-C346221 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence blot of A549 cell using NSL1 antibody.

Anti-NSL1 Antibody (aa150-200) - LS-C79314 from LifeSpan Bioscience

Prices	$545.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NSL1 Antibody (aa1-275) - LS-C185620 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence of paraformaldehyde-fixed HeLa using DC8 antibody at 1:200 dilution.

DC8 Antibody - NB100-2575 from Novus Biologicals

Prices	$369.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: DC8 Antibody [NB100-2575] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

DC8 Antibody - NBP1-32281 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

DC8 Antibody - NBP2-13678 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: DC8 Antibody [NBP2-13678] Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.

DC8 Antibody - H00025936-A01 from Novus Biologicals

Host	Mouse
Clonality	Polyclonal

Anti-NSL1 antibody produced in rabbit - SAB2700030 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-NSL1: <B>Cat. No. SAB2700030</B>: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8 antibody at 1:200 dilution.

Anti-NSL1 antibody produced in rabbit - SAB2107688 from Sigma-Aldrich

Prices	$341.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-NSL1: <B>Cat. No. SAB2107688</B>: Western blot analysis of NSL1 in HeLa with NSL1 (NSL1, MIND kinetochore complex component, homolog, S. cerevisiae) antibody, against the C terminal of NSL1 (50 μg) antibody at 1.0 μg/mL.

NSL1 antibody - C-terminal region (ARP62184_P050) - ARP62184_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

DC8 antibody - orb73633 from Biorbyt

Prices	$723.00
Sizes	100 μl
Host	Rabbit
Clonality	Polyclonal

Western Blot analysis of A:HepG2 whole cell lysate using DC8 antibody

NSL1 Polyclonal Antibody - A7089 from ABclonal

Prices	$130.00, $220.00, $380.00
Sizes	50 µl, 100 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence analysis of A549 cell using NSL1 antibody.

NSL1 antibody (OAGA00311) - OAGA00311 from Aviva Systems Biology

Prices	$340.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

NSL1 antibody - H00025936-A01 from Acris Antibodies

Prices	$300.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Rabbit Anti-Human DC8 - 107-10452 from RayBiotech

Host	Rabbit

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