POU3F4

Gene Symbol	POU3F4
Entrez Gene	5456
Alt Symbol	BRAIN-4, BRN-4, BRN4, DFN3, DFNX2, OCT-9, OTF-9, OTF9
Species	Human
Gene Type	protein-coding
Description	POU class 3 homeobox 4
Other Description	POU domain, class 3, transcription factor 4\|brain-specific homeobox/POU domain protein 4\|octamer-binding transcription factor 9
Swissprots	P49335 B2RC71 Q99410 Q5H9G9
Accessions	EAW98577 P49335 AK314967 BAG37468 BC141561 AAI41562 BC146551 AAI46552 X82324 CAA57767 NM_000307 NP_000298
Function	Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.
Subcellular Location	Nucleus.
Tissue Specificity	Brain specific.

POU3F4 (C-18) - sc-69591 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

POU3F4 (G-15) - sc-69592 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

POU3F4 (G-15): sc-69592. Western blot analysis of POU3F4 expression in rat hippocampus (A), rat cerebellum (B), rat brain (C) and mouse brain (D) tissue extracts and MIA PaCa-2 (E) and Jurkat (F) whole cell lysates.

POU3F4 (H-62) - sc-292121 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

POU3F4 (S-17) - sc-69594 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

POU3F4 (S-17): sc-69594. Western blot analysis of POU3F4 expression in rat cerebellum (A) and rat brain (B) tissue extracts.

POU3F4 (K-21) - sc-133921 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

POU3F4 (K-21): sc-133921. Western blot analysis of POU3F4 expression in Hep G2 whole cell lysate.

Anti-BRN4 antibody - ab104562 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-BRN4 antibody (ab104562) at 1 µg/mlLane 1 : Rat Hippocampus Tissue LysateLane 2 : Cerebellum Rat Tissue LysateLane 3 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-BRN4 antibody - ab167536 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-BRN4 antibody (ab167536) at 1/500 dilutionLane 1 : BRN4 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse IgG HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-BRN4 antibody - ab23339 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-BRN4 antibody (ab23339) at 0.4 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900) with 5% skim milk/PBS bufferSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721)

Anti-POU3F4 Antibody (C-Terminus) - LS-C30870 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

BRN4 Antibody - NBP1-89934 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: BRN4 Antibody [NBP1-89934] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

BRN4 Antibody - H00005456-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: BRN4 Antibody [H00005456-B01] - analysis of POU3F4 expression in transfected 293T cell line by POU3F4 MaxPab polyclonal antibody. Lane 1: POU3F4 transfected lysate (39.71 KDa). Lane 2: Non-transfected lysate.

POU3F4 Polyclonal Antibody - bs-8202R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with POU3F4 Polyclonal Antibody, Unconjugated (bs-8202R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Anti-POU3F4 antibody produced in rabbit - HPA031984 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-POU3F4: <B>Cat. No. HPA031984</B>: Immunohistochemical staining of human lateral ventricle shows moderate nuclear positivity in neuronal cells.

Anti-POU3F4 antibody produced in rabbit - AV100907 from Sigma-Aldrich

Prices	$277.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV100907</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.3-0.5 μg/mL in cell type HepG2

Anti-BRN4 Antibody - AC11-0101 from Abcore

Prices	$325.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

POU3F4 antibody - 25114-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25114-1-AP( POU3F4 Antibody) at dilution of 1:300

POU3F4 antibody - H00005456-B01 from Acris Antibodies

Prices	$360.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot analysis of POU3F4 expression in transfected 293T cell line (H00005456-T01) by POU3F4 MaxPab polyclonal antibody. Lane 1: POU3F4 transfected lysate(39.71 KDa). Lane 2: Non-transfected lysate.

POU3F4 Antibody - 31-222 from ProSci

Prices	$250.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Antibody used in WB on Human HepG2 at 0.3-0.5 ug/ml.

POU3F4 antibody - 25114-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 33 μg/150μl
Host	Rabbit
Clonality	Polyclonal

POU3F4-Specific antibody - 19000-1-AP from Proteintech Group

Host	Rabbit
Clonality	Polyclonal

SPTBN5 Polyclonal Antibody - PA003412-C18906 from Syd Labs

Prices	$150.00, $250.00
Sizes	50 µg, 100 µg
Host	Rabbit
Clonality	Polyclonal

POU3F4 Antibody - GWB-C38429 from Genway Biotech

Prices	$268.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Rabbit Anti-Human POU3F4 Polyclonal Antibody - DPABH-06673 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

Anti-POU3F4 (aa 313-361) polyclonal antibody - DPABH-15241 from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

Anti-POU3F4 monoclonal antibody - DCABH-201286 from Creative Diagnostics

Host	Rabbit
Clonality	Monoclonal

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