SMAGP

Gene Symbol	SMAGP
Entrez Gene	57228
Alt Symbol	hSMAGP
Species	Human
Gene Type	protein-coding
Description	small cell adhesion glycoprotein
Other Description	small cell transmembrane and glycosylated protein\|small trans-membrane and glycosylated protein\|small transmembrane and glycosylated protein
Swissprots	A6NIL5 Q0VAQ4
Accessions	EAW58183 EAW58184 EAW58185 Q0VAQ4 AF091087 AAC72956 AK001839 BC001400 BC003379 AAH03379 BC008712 AAH08712 BC045658 BC120965 AAI20966 BC120966 AAI20967 BG740791 CA776511 CR456943 CAG33224 NM_001031628 NP_001026798 NM_001033873 NP_001029045
Function	May play a role in epithelial cell-cell contacts. May play a role in tumor invasiveness and metastasis formation. {ECO:0000269\|PubMed:15986429}.
Subcellular Location	Cell membrane; Single-pass type III membrane protein. Cytoplasmic vesicle membrane; Single-pass type III membrane protein. Note=Predominantly on lateral parts of the membrane, at cell-cell epithelial junctions. Detected on cytoplasmic membranes in undifferentiated tumors.
Tissue Specificity	Detected in breast, endometrium, colon and biliary tract. Detected in polarized epithelial structures characterized by cell-cell adhesion (at protein level). {ECO:0000269\|PubMed:15021913}.

SMAGP (K-15) - sc-248655 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

SMAGP (P-17) - sc-248654 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-SMAGP antibody - ab104059 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SMAGP antibody (ab104059) at 1/100 dilution + K562 cell line lysate at 35 µg

Anti-SMAGP antibody - C-terminal - ab198711 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling SMAGP with ab198711 at 1/40 dilution.

SMAGP Antibody - AF3959 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

SMAGP Antibody - NBP1-81686 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: SMAGP Antibody [NBP1-81686] - Staining of human placenta shows cytoplasmic and membranous positivity in trophoblastic cells.

SMAGP Polyclonal Antibody - bs-5844R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-SMAGP antibody produced in rabbit - HPA039711 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-SMAGP: <B>Cat. No. HPA039711</B>: Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-SMAGP (81-95) antibody produced in rabbit - SAB1102486 from Sigma-Aldrich

Prices	$298.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG<SUP>®</SUP>-SMAGP fusion protein was separated on SDS-PAGE, blotted with Anti-SMAGP (81-95) (<B>Cat. No. SAB1102486</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + SMAGP immunizing peptide (human, 81-95)<BR/>3. Negative control (only secondary antibody)<BR/>4. Anti-FLAG<SUP>®</SUP> antibody (<B>Cat. No. F7425</B>), working concentration 2 μg/mL

ANTI-SMAGP (CENTER) antibody produced in rabbit - SAB1307188 from Sigma-Aldrich

Prices	$326.00
Sizes	40 tst
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-SMAGP (Center): <B>Cat. No. SAB1307188</B>: SMAGP Antibody (Center)（Cat. #AP5271c）western blot analysis in K562 cell line lysates (35 μg/lane).

SMAGP Antibody - middle region (OAAB02688) - OAAB02688 from Aviva Systems Biology

Prices	$289.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

SMAGP-Antibody-Center - AP5271c from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	80 µl, 400 µl
Host	Rabbit
Clonality	Polyclonal

SMAGP Antibody (Center)（Cat. #AP5271c）western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the SMAGP antibody detected the SMAGP protein (arrow).

Human SMAGP Antibody - AF3959 from R&D Systems

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat

Detection of Human SMAGP by Western Blot. Western blot shows lysates of human lung tissue, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMAGP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019 ). A specific band was detected for SMAGP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .

Human SMAGP Affinity Purified Polyclonal Ab, Goat IgG - AF3959-SP from R&D Systems

Host	Goat
Clonality	Polyclonal

Rat SMAGP Antibody - AF3967 from R&D Systems

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat

Rat SMAGP Affinity Purified Polyclonal Ab, Goat IgG - AF3967-SP from R&D Systems

Host	Goat
Clonality	Polyclonal

SMAGP Antibody - abx032465 from Abbexa

Host	Rabbit
Clonality	Polyclonal

SMAGP antibody - 14998-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Hela cell were subjected to SDS PAGE followed by western blot with 14998-1-AP(LOC57228 Antibody) at dilution of 1:800

SMAGP antibody - 14998-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 27 μg/150μl
Host	Rabbit
Clonality	Polyclonal

COLO 320 cells were subjected to SDS PAGE followed by western blot with 14998-1-AP(LOC57228 antibody) at dilution of 1:600

SMAGP Antibody - 37954 from Signalway Antibody

Prices	$279.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #37954 at dilution 1/40.

LOC57228, Polyclonal Antibody - MBS2518047 from MyBioSource

Prices	$195.00, $270.00, $435.00
Sizes	60 µl, 120 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

SMAGP Polyclonal Antibody - ESAP13918 from Elabscience

Prices	$296.00, $485.00
Sizes	120 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of Human prostate cancer using SMAGP Polyclonal Antibody at dilution of 1:40

SMAGP Polyclonal Antibody - ESAP17221 from Elabscience

Prices	$296.00, $485.00
Sizes	120 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of Human brain using SMAGP Polyclonal Antibody at dilution of 1:35

LOC57228 Antibody - EPP14996 from Elabscience

Prices	$296.00, $485.00
Sizes	120 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

SMAGP Polyclonal Antibody - PA5-25195 from Thermo Fisher Scientific

Prices	$349.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human breast carcinoma using a SMAGP polyclonal antibody (Product ## PA5-25195), followed by HRP-conjugated secondary antibody and DAB staining.

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