PRNP

Gene Symbol	PRNP
Entrez Gene	281427
Alt Symbol	AltPrP, PrP
Species	Bovine
Gene Type	protein-coding
Description	prion protein
Other Description	major prion protein\|major scrapie-associated fibril protein 1\|prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia)\|prion protein PrP\|prion protein precursor PrP\|prion protein variant a\|prion protein variant b
Swissprots	Q6UL06 Q6UL03 Q0VD57 Q7YRN3 Q01880 P10279 Q8MJI7 Q6UL05 Q5UJJ5 Q5UK71 Q5UK69 Q6UL04 A1YVV9 Q5UJM6 F7VJQ2
Accessions	AAD19998 AAM73856 AAO92929 AAP84097 AAQ64645 AAQ64646 AAQ64647 AAQ64648 AAQ64649 AAQ64650 AAQ94042 AAQ94043 AAQ94044 AAQ94045 AAQ94046 AAQ94047 AAQ94048 AAQ94049 AAQ94050 AAQ94051 AAV30252 AAV30253 AAV30255 AAV30256 AAV30257 AAV30258 AAV30259 AAV30260 AAV30261 AAV30262 AAV30265 AAV30266 AAV30267 AAV30268 AAV30269 AAV30270 AAV30271 AAV30272 AAV30273 AAV30274 AAV30275 AAV30276 AAV30277 AAV30278 AAV30279 AAV30280 AAV30281 AAV30282 AAV30283 AAV30284 AAV30285 AAV30286 AAV30287 AAV30288 AAV30289 AAV30290 AAV30291 AAV30292 AAV30293 AAV30295 AAV30296 AAV30299 AAV30300 AAV30301 AAV30302 AAV30303 AAV30304 AAV30305 AAV30310 AAV30311 AAV30312 AAV30313 AAV30314 AAV30315 AAV30316 AAV30317 AAV30318 AAV30319 AAV30320 AAV30321 AAV30332 AAV30337 AAV30338 AAV30339 AAV30340 AAV30341 AAV30342 AAV30347 AAV30348 AAV30351 AAV30352 AAV30353 AAV30354 AAV30355 AAV30356 AAV30357 AAV30358 AAV30359 AAV30360 AAV30361 AAV30362 AAV30363 AAV30364 AAV30365 AAV30366 AAV30367 AAV30368 AAV30369 AAV30370 AAV30371 AAV30372 AAV30373 AAV30374 AAV30375 AAV30376 AAV30377 AAV30378 AAV30379 AAV30380 AAV30381 AAV30388 AAV30389 AAV30390 AAV30391 AAV30392 AAV30395 AAV30398 AAV30401 AAV30402 AAV30403 AAV30404 AAV30407 AAV30408 AAV30409 AAV30410 AAV30411 AAV30412 AAV30413 AAV30414 AAV30415 AAV30416 AAV30417 AAV30418 AAV30419 AAV30420 AAV30429 AAV30430 AAV30431 AAV30432 AAV30433 AAV30434 AAV30435 AAV30436 AAV30437 AAV30438 AAV30439 AAV30440 AAV30441 AAV30442 AAV30443 AAV30444 AAV30447 AAV30448 AAV30449 AAV30450 AAV30451 AAV30452 AAV30455 AAV30456 AAV30457 AAV30458 AAV30459 AAV30460 AAV30461 AAV30462 AAV30463 AAV30464 AAV30469 AAV30470 AAV30471 AAV30474 AAV30475 AAV30476 AAV30477 AAV30478 AAV30479 AAV30480 AAV30481 AAV30482 AAV30483 AAV30484 AAV30485 AAY21624 AAZ06807 ABB51165 ABC61819 ABE02801 ABE02802 ABJ52640 ABJ52641 ABJ52642 ABJ52644 ABJ52645 ABJ52646 ABJ52647 ABJ52648 ABJ52649 ABJ52650 ABJ52651 ABJ52652 ABJ52653 ABJ52654 ABJ52655 ABJ52656 ABJ52657 ABJ52658 ABJ52659 ABJ52660 ABJ52661 ABJ52662 ABJ52663 ABJ52664 ABJ52665 ABJ52666 ABJ52667 ABJ52668 ABJ52669 ABJ52670 ABJ52671 ABJ52672 ABJ52673 ABJ52675 ABJ52676 ABJ52677 ABJ52678 ABJ52679 ABJ52680 ABJ52681 ABJ52682 ABJ52683 ABJ52684 ABJ52685 ABJ52686 ABJ52687 ABJ52688 ABJ52689 ABJ52690 ABJ52691 ABJ52692 ABJ52693 ABJ52694 ABJ52695 ABJ52696 ABJ52697 ABJ52698 ABJ52699 ABJ52700 ABL75501 ABR92636 ABS76306 ABS76307 ABS76308 ABS76309 ABS76310 ABS76311 ABS76312 ABS76313 ABS76314 ABS76315 ABS76316 ABS76317 ABU97798 ABU97799 ABU97800 ABU97801 ABU97802 ABU97803 ABU97804 ABU97805 ABU97806 ABU97807 ABU97808 ABU97809 ABU97810 ABU97811 ABU97812 ABU97813 ABU97814 ABU97815 ABU97816 ABU97817 ABU97818 ABU97819 ABU97820 ABU97821 ABU97822 ABU97823 ABU97824 ABU97825 ABU97826 ABU97827 ABU97828 ABU97829 ABU97830 ABU97831 ABU97832 ABU97833 ABU97834 ABU97835 ABU97836 ABU97837 ABU97838 ABU97839 ABU97840 ABU97841 ABU97842 ABU97843 ABU97844 ABU97845 ABU97846 ABU97847 ABU97848 ABU97849 ABU97850 ABU97851 ABU97852 ABU97853 ABU97854 ABU97855 ABU97856 ABU97857 ABU97858 ABU97859 ABU97860 ABU97861 ABU97862 ABU97863 ABU97864 ABU97865 ABU97866 ABU97867 ABU97868 ABU97869 ABU97870 ABU97871 ABU97872 ABU97873 ABU97874 ABU97875 ABU97876 ABU97877 ABU97878 ABU97879 ABU97880 ABU97881 ABU97882 ABU97883 ABU97884 ABU97885 ABU97886 ABU97887 ABU97888 ABU97889 ABU97890 ABU97891 ABU97892 ABU97893 ABU97894 ABU97895 ABU97896 ABU97897 ABU97898 ABU97899 ABU97900 ABU97901 ABU97902 ABU97903 ABU97904 ABU97905 ABU97906 ABU97907 ABU97908 ABU97909 ABU97910 ABU97911 ABU97912 ABU97913 ABU97914 ABU97915 ABU97916 ABU97917 ABU97918 ABU97919 ABU97920 ABU97921 ABU97922 ABU97923 ABU97924 ABU97925 ABU97926 ABU97927 ABU97928 ABU97929 ABU97930 ABU97931 ABU97932 ABU97933 ABU97934 ABU97935 ABU97936 ABU97937 ABU97938 ABU97939 ABU97940 ABU97941 ABU97942 ABU97943 ABU97944 ABU97945 ABU97946 ABU97947 ABU97948 ABU97949 ABU97950 ABU97951 ABU97952 ABU97953 ABU97954 ABU97955 ABU97956 ABU97957 ABU97958 ABU97959 ABU97960 ABU97961 ABU97962 ABU97963 ABU97964 ABU97965 ABU97966 ABU97967 ABU97968 ABU97969 ABU97970 ABU97971 ABU97972 ABU97973 ABU97974 ABU97975 ABU97976 ABU97977 ABU97978 ABU97979 ABU97980 ABU97981 ABU97982 ABU97983 ABU97984 ABU97985 ABU97986 ABU97987 ABU97988 ABU97989 ABU97990 ABU97991 ABU97992 ABU97993 ABU97994 ABU97995 ABU97996 ABU97997 ABU97998 ABU97999 ABU98000 ABU98001 ABU98002 ABU98003 ABU98004 ABU98005 ABU98006 ABU98007 ABU98008 ABU98009 ABU98010 ABU98011 ABU98012 ABU98013 ABU98014 ABU98015 ABU98016 ABU98017 ABU98018 ABU98019 ABU98020 ABU98021 ABU98022 ABU98023 ABU98024 ABU98025 ABU98026 ABU98027 ABU98028 ABU98029 ABU98030 ABU98031 ABU98032 ABU98033 ABU98034 ABU98035 ABU98036 ABU98037 ABU98038 ABU98039 ABU98040 ABU98041 ABU98042 ABU98043 ABU98044 ABU98045 ABU98046 ABU98048 ABU98049 ABU98050 ABU98051 ABU98052 ABU98053 ABU98054 ABU98055 ABU98056 ABU98057 ABU98058 ABU98059 ABU98060 ABU98061 ABU98062 ABU98063 ABU98064 ABU98065 ABU98066 ABU98067 ABU98068 ABU98069 ABU98070 ABU98071 ABU98072 ABU98073 ABU98074 ABU98075 ABU98076 ABU98077 ABU98078 ABU98079 ABU98080 ABU98081 ABU98082 ABU98083 ABU98084 ABU98085 ABU98086 ABU98087 ABU98088 ABU98089 ABU98090 ABU98091 ABU98092 ABU98093 ABU98094 ABU98095 ABU98096 ABU98097 ABU98098 ABU98099 ABU98100 ABU98101 ABU98102 ABU98103 ABU98104 ABU98105 ABU98106 ABU98107 ABU98108 ABU98109 ABU98110 ABU98111 ABU98112 ABU98113 ABU98114 ABU98115 ABU98116 ABU98117 ABU98118 ABU98119 ABU98120 ABU98121 ABU98122 ABU98123 ABU98124 ABU98125 ABU98126 ABU98127 ABU98128 ABU98129 ABU98130 ABU98131 ABU98132 ABU98133 ABU98134 ABU98135 ABU98136 ABU98137 ABU98138 ABU98139 ABU98140 ABU98141 ABU98142 ABU98143 ABU98144 ABU98145 ABU98146 ABU98147 ABU98148 ABU98149 ABU98150 ABU98151 ABU98152 ABU98153 ABU98154 ABU98155 ABU98156 ABU98157 ABU98158 ABU98159 ABU98160 ABU98161 ABU98162 ABU98163 ABU98164 ABU98165 ABU98166 ABU98167 ABU98168 ABU98169 ABU98170 ABU98171 ABU98172 ABU98173 ABU98174 ABU98175 ABU98176 ABU98177 ABU98178 ABU98179 ABU98180 ABU98181 ABU98182 ABU98183 ABU98184 ABU98185 ABU98186 ABU98187 ABU98188 ABU98189 ABU98190 ABU98191 ABU98192 ABU98193 ABU98194 ABU98195 ABU98196 ABU98197 ABU98198 ABU98199 ABU98200 ABU98201 ABU98202 ABU98203 ABU98204 ABU98205 ABU98206 ABU98207 ABU98208 ABU98209 ABU98210 ABU98211 ABU98212 ABU98213 ABU98214 ABU98215 ABU98216 ABU98217 ABU98218 ABU98219 ABU98220 ABU98221 ABU98222 ABU98223 ABU98224 ABU98225 ABU98226 ABU98227 ABU98228 ABU98229 ABU98230 ABU98231 ABU98232 ABU98233 ABU98234 ABU98235 ABU98236 ABU98237 ABU98238 ABU98239 ABU98240 ABU98241 ABU98242 ABU98243 ABU98244 ABU98245 ABU98246 ABU98247 ABU98248 ABU98249 ABU98250 ABU98251 ABU98252 ABU98253 ABU98254 ABU98255 ABU98256 ABU98257 ABU98258 ABU98259 ABU98260 ABU98261 ABU98262 ABU98263 ABU98264 ABU98265 ABU98266 ABU98267 ABU98268 ABU98269 ABU98270 ABU98271 ABU98272 ABU98273 ABU98274 ABU98275 ABU98276 ABU98277 ABU98278 ABU98279 ABU98280 ABU98281 ABU98282 ABU98283 ABU98284 ABU98285 ABU98286 ABU98287 ABU98288 ABU98289 ABU98290 ABU98291 ABV71907 ABV71908 ABV71909 ABV71910 ABW89572 ABW89573 ABW89574 ABW89575 ABW89576 ABW89577 ABW89578 ABW89579 ABW89580 ABW89581 ABW89582 ABW89583 ABW89584 ABW89585 ACE73992 ACE73993 ACE73994 ACE73995 ACE73996 ACE73997 ACE73998 ACE73999 ACE74000 ACE74001 ACE74002 ACE74003 ACE74004 ACE74005 ACE74006 ACT98557 ACT98558 ACT98559 ACT98561 ACT98564 ACT98565 ACT98566 ACT98567 ACT98568 ACT98570 ACT98573 ACT98574 ACT98575 ACT98576 ACT98577 ACT98578 ACT98581 ACT98582 ACT98583 ACT98584 ACT98585 ACT98586 ACT98587 ACT98589 ACT98593 ACT98594 ACT98595 ACT98596 ACT98597 ACT98598 ACT98599 ACT98600 ACT98601 ACT98602 ACT98603 ACT98605 ACT98606 ACT98607 BAE78583 BAE78586 BAI50004 BAI50005 BAM66564 BAM66567 CAA39368 CAC37367 DAA23426 DAA34791 F7VJQ2 P10279 AB001468 BAA19253 AF517842 AAM66709 AY585239 AAT09128 BC119821 AAI19822 D10612 BAA01467 GU817011 ADX21067 S55629 AAB25514 NM_001271625 NP_001258554 NM_001271626 NP_001258555 NM_181015 NP_851358
Function	May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity). {ECO:0000250}.
Subcellular Location	Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. Golgi apparatus {ECO:0000250}. Note=Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU(2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis (By similarity). {ECO:0000250}.
Top Pathways	Prion diseases

Anti-Prion protein PrP antibody - ab6664 from Abcam

Prices	$379.00
Sizes	50 µl
Host	Goat
Clonality	Polyclonal

CJD brain section showing stained prion plaque using ab6664 at 1:200

Anti-Prion protein PrP antibody - ab703 from Abcam

Prices	$379.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Lane 1: MW standardLane 2: wild-type mice (PrPc)Lane 3: CDI/PrPsc (PK-)Lane 4: CDI/PrPsc (PK+), Lane 5: PrP 0/0 mice CDI = clinically ill CD-1 mice, PK = digested with Proteinase K.Dr. M. Glatzel, Institut of Neuropathology, University Hospital of Zürich, Switzerland

Anti-Prion protein PrP antibody - ab50429 from Abcam

Prices	$376.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

Anti-Prion protein PrP antibody (ab50429) at 0.3 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).

Anti-Prion protein PrP antibody - ab93273 from Abcam

Prices	$359.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

Anti-Prion protein PrP antibody - ab22366 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Mouse
Clonality	Polyclonal

Anti-Prion protein PrP antibody - ab89750 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Prion protein PrP antibody (ab89750) at 1/5000 dilutionLane 1 : Recombinant Prion protein PrP from CowLane 2 : Recombinant Prion protein PrP from HumanLane 3 : Recombinant Prion protein PrP from SheepLane 4 : Recombinant Prion protein PrP from DeerSecondaryHRP-conjugated Goat anti-Mouse IgG/M antibody

Anti-Prion protein PrP antibody - ab89751 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Prion protein PrP antibody (ab89751) at 1/5000 dilutionLane 1 : Recombinant Prion protein PrP from CowLane 2 : Recombinant Prion protein PrP from HumanLane 3 : Recombinant Prion protein PrP from SheepLane 4 : Recombinant Prion protein PrP from DeerSecondaryHRP-conjugated Goat anti-Mouse IgG/M

Anti-Prion protein PrP antibody [1E5 / G6] - ab2879 from Abcam

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	1E5 / G6

ab2879 at a 1 µg/ml concentration staining ~23 kDa (mature) and ~28 kDa (pro-peptide) native bovine prion protein from brain (M. oblongata; 100 µg comprising 10% homogenised brain, diluted 1:10 in buffer, 10µl loaded) by Western blot (non-reducing conditions).

Anti-Prion protein PrP antibody [1E5 / G6] (HRP) - ab3408 from Abcam

Prices	$379.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1E5 / G6

Anti-Prion protein PrP antibody [1E5 / G6] (HRP) (ab3408) at 1 µg/ml + native bovine prion protein from brain (M. oblongata)Performed under non-reducing conditions.Observed band size : 19 kDa (why is the actual band size different from the predicted?)Additional bands at : 27 kDa (possible glycosylated form),30 kDa (possible glycosylated form).

Anti-Prion protein PrP antibody [3B8 / D5] - ab2881 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3B8 / D5

ab2881 at a 4 µg/ml concentration staining ~23 kDa (mature) and ~28 kDa (pro-peptide) native bovine prion protein from brain (M. oblongata; 100 µg comprising 10% homogenised brain, diluted 1:10 in buffer, 10µl loaded) by Western blot (non-reducing conditions).

Anti-Prion protein PrP antibody [5121] - ab156664 from Abcam

Prices	$372.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	5121

Anti-Prion protein PrP antibody [5B9] (Biotin) - ab61333 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	5B9

All lanes : Anti-Prion protein PrP antibody [5B9] (Biotin) (ab61333) at 2 µg/mlLane 1 : Recombinant human prion proteinLane 2 : Recombinant bovine prion proteinLysates/proteins at 1 µg per lane.SecondaryHorseradish peroxidase conjugated goat anti-mouse IgG antibodyPerformed under reducing conditions.

Anti-Prion protein PrP antibody [5C4] - ab22256 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	5C4

Anti-Prion protein PrP antibody [7B6 / D2] - ab2882 from Abcam

Prices	$378.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	7B6 / D2

ab2882 at a 1 µg/ml concentration staining ~23 kDa (mature) and ~28 kDa (pro-peptide) native bovine prion protein from brain (M. oblongata; 100 µg comprising 10% homogenised brain, diluted 1:10 in buffer, 10µl loaded) by Western blot (non-reducing conditions).

Anti-Prion protein PrP antibody [7B6 / D2] (HRP) - ab3410 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	7B6 / D2

Anti-Prion protein PrP antibody [7D9] - ab14219 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	7D9

Anti-Prion protein PrP antibody [8H4] - ab61409 from Abcam

Prices	$381.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	8H4

All lanes : Anti-Prion protein PrP antibody [8H4] (ab61409) at 1/2500 dilutionLane 1 : Wild type mouse hippocampus whole tissue lysateLane 2 : Prnp-/- mouse hippocampus whole tissue lysateLysates/proteins at 30 µg per lane.SecondaryHRP-conjugated sheep anti-mouse IgG monoclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Prion protein PrP antibody [F89/160.1.5] - ab2777 from Abcam

Prices	$379.00
Sizes	200 µg
Host	Mouse
Clonality	Monoclonal
Clone	F89/160.1.5

Overlay histogram showing SH-SH5Y cells stained with ab2777 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2777, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Prion protein PrP antibody [T16-R] - ab136919 from Abcam

Prices	$378.00
Sizes	50 µl
Host	Rabbit
Clonality	Monoclonal
Clone	T16-R

All lanes : Anti-Prion protein PrP antibody [T16-R] (ab136919) at 1/1000 dilutionLane 1 : Mouse brain extractLane 2 : HEK293 cells transfected with Human Prion protein PrP gene Lane 3 : HEK293 cells transfected with empty vectorLysates/proteins at 200 µg per lane.

Anti-Prion protein PrP antibody [T325] - ab77278 from Abcam

Prices	$372.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	T325

Anti-Prion protein PrP antibody - Azide free - ab23973 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Prion protein Antibody - NB100-56604 from Novus Biologicals

Prices	$279.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: Prion protein Antibody [NB100-56604] - analysis of BSE in (A) recombinant fusion protein containing amino acids 142-148 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml.

Prion protein Antibody - NB100-56605 from Novus Biologicals

Prices	$99.00, $229.00
Sizes	25 µg, 100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: Prion protein Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.

Prion protein Antibody - NB100-56606 from Novus Biologicals

Prices	$279.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: Prion protein Antibody [NB100-56606] - analysis of BSE in (A) recombinant fusion protein containing amino acids 217-229 and (B) fusion partner without these amino acids, using this antibody. 5 ug/ml.

Monoclonal Anti-Prion Protein antibody produced in mouse - P6488 from Sigma-Aldrich

Prices	$422.50
Sizes	200 µg
Host	Mouse
Clonality	Monoclonal
Clone	F89/160.1.5

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