PNO1

Gene Symbol	PNO1
Entrez Gene	56902
Alt Symbol	KHRBP1, RRP20
Species	Human
Gene Type	protein-coding
Description	partner of NOB1 homolog (S. cerevisiae)
Other Description	KH-type RNA-binding protein 1\|RNA binding protein\|RNA-binding protein PNO1
Swissprots	Q8WVB8 Q53G13 A8K6Q0 Q9NRX1
Accessions	AAY14714 EAW99879 Q9NRX1 AF164799 AAF80763 AF349314 AAK29434 AK223118 BAD96838 AK225641 AK291715 BAF84404 AK313204 BAG36020 BC008304 AAH08304 BC018152 AAH18152 L48692 NM_020143 NP_064528
Subcellular Location	Nucleus, nucleolus {ECO:0000269\|PubMed:15497447}.
Tissue Specificity	Expressed in liver, lung, spleen and kidney. Weakly expressed in thymus, testis and ovary. Weakly or not expressed in heart, brain, skeletal muscle, placenta, pancreas, prostate, small intestine, colon and peripheral blood leukocytes. {ECO:0000269\|PubMed:15497447}.

PNO1 (D-14) - sc-133263 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

PNO1 (D-14): sc-133263. Western blot analysis of PNO1 expression in non-transfected 293T: sc-117752 (A), mouse PNO1 transfected 293T: sc-125837 (B) and K-562 (C) whole cell lysates.

PNO1 (K-14) - sc-133265 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

PNO1 (K-14): sc-133265. Western blot analysis of PNO1 expression in K-562 whole cell lysate.

Anti-PNO1 antibody - ab140148 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PNO1 antibody (ab140148) at 1/1000 dilution + HeLa cell lysate

Anti-PNO1 antibody - ab69156 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-PNO1 antibody (ab69156) at 1/500 dilutionLane 1 : PNO1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-PNO1 Antibody (aa31-80) - LS-C145844 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PNO1 antibody LS-C145844 Western blot of HeLa Cell lysate. Antibody concentration 1 ug/ml.

Anti-PNO1 Antibody - LS-C332588 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using PNO1 antibody.

Anti-PNO1 Antibody (aa13-187) - LS-C179090 from LifeSpan Bioscience

Prices	$255.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PNO1 Antibody - NBP2-30415 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PNO1 Antibody [NBP2-30415] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp

PNO1 Antibody - H00056902-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: PNO1 Antibody [H00056902-B01] - analysis of LOC56902 expression in transfected 293T cell line by LOC56902 MaxPab polyclonal antibody (B01). Lane 1: LOC56902 transfected lysate (27.90 KDa). Lane 2: Non-transfected lysate.

PNO1 Antibody - H00056902-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: PNO1 Antibody [H00056902-B01P] - Analysis of PNO1 expression in transfected 293T cell line by PNO1 polyclonal antibody. Lane 1: LOC56902 transfected lysate(27.72 KDa). Lane 2: Non-transfected lysate.

Anti-PNO1 antibody produced in rabbit - HPA056928 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-PNO1: <B>Cat. No. HPA056928</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Anti-PNO1 antibody produced in mouse - SAB1407751 from Sigma-Aldrich

Prices	$347.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-PNO1: <B>Cat. No. SAB1407751</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~27.7 kDa using PNO1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-PNO1 (73-85) antibody produced in rabbit - SAB1102471 from Sigma-Aldrich

Prices	$298.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PNO1 was separated on SDS-PAGE, blotted with Anti-PNO1 (73-85) (<B>Cat. No. SAB1102471</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + PNO1 immunizing peptide (human, 73-85)

PNO1 antibody - N-terminal region (ARP62587_P050) - ARP62587_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PNO1 antibody - 70R-19382 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

PNO1 Polyclonal Antibody - A3467 from ABclonal

Prices	$200.00, $480.00
Sizes	50 µg, 200 µg
Host	Rabbit
Clonality	Polyclonal

PNO1 antibody - orb78411 from Biorbyt

Prices	$425.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of formalin-fixed paraffin embedded fetal spleen showing nuclear staining using PNO1 antibody

PNO1 Antibody - abx036283 from Abbexa

Host	Rabbit
Clonality	Polyclonal

PNO1 Antibody - abx002494 from Abbexa

Host	Rabbit
Clonality	Polyclonal

PNO1 antibody - 21059-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21059-1-AP(PNO1 antibody) at dilution of 1:500

PNO1 antibody - H00056902-B01 from Acris Antibodies

Prices	$360.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot analysis of PNO1 expression in transfected 293T cell line (H00056902-T01) by PNO1 MaxPab polyclonal antibody.Lane 1: LOC56902 tr

PNO1 antibody - H00056902-B01P from Acris Antibodies

Prices	$360.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-PNO1, Polyclonal Antibody - 25-A3467 from ARP American Research Products

Prices	$299.00, $449.00
Sizes	100 µg, 200 µg
Host	Rabbit
Clonality	Polyclonal

PNO1 antibody - STJ26324 from St John's Laboratory Ltd

Host	Rabbit
Clonality	Polyclonal

PNO1 antibody - 21059-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 21 μg/150μl
Host	Rabbit
Clonality	Polyclonal

L02 cells were subjected to SDS PAGE followed by western blot with 21059-1-AP(PNO1 antibody) at dilution of 1:300

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