Gene Symbol | Blm |
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Entrez Gene | 12144 |
Alt Symbol | - |
Species | Mouse |
Gene Type | protein-coding |
Description | Bloom syndrome, RecQ helicase-like |
Other Description | Bloom syndrome homolog|Bloom syndrome protein homolog|mBLM|recQ helicase homolog |
Swissprots | O88198 O88700 |
Accessions | EDL06982 EDL06983 O88700 AB008674 BAA32001 AK137022 AK139975 AK157898 AK164605 BB614580 BB655193 BC157095 AAI57096 Z98263 CAB10933 NM_001042527 NP_001035992 NM_007550 NP_031576 |
Function | Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). {ECO:0000269|PubMed:9840919}. |
Subcellular Location | Nucleus {ECO:0000250}. Note=Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR- dependent manner (By similarity). {ECO:0000250}. |
Tissue Specificity | Highly expressed in testis 12-14 days after birth (corresponding to the pachytene phase) and at much lower levels in brain, heart, liver, lung, thymus, kidney and spleen. {ECO:0000269|PubMed:9655940}. |
Top Pathways | Homologous recombination, Fanconi anemia pathway |
BLM CRISPR/Cas9 KO Plasmid (m) - sc-419336 from Santa Cruz Biotechnology
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BLM HDR Plasmid (m) - sc-419336-HDR from Santa Cruz Biotechnology
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BLM Double Nickase Plasmid (m) - sc-419336-NIC from Santa Cruz Biotechnology
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BLM Double Nickase Plasmid (m2) - sc-419336-NIC-2 from Santa Cruz Biotechnology
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BLM CRISPR Activation Plasmid (m) - sc-419336-ACT from Santa Cruz Biotechnology
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BLM CRISPR Activation Plasmid (m2) - sc-419336-ACT-2 from Santa Cruz Biotechnology
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BLM Lentiviral Activation Particles (m) - sc-419336-LAC from Santa Cruz Biotechnology
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BLM Lentiviral Activation Particles (m2) - sc-419336-LAC-2 from Santa Cruz Biotechnology
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BLM siRNA (m) - sc-29809 from Santa Cruz Biotechnology
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BLM shRNA Plasmid (m) - sc-29809-SH from Santa Cruz Biotechnology
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BLM shRNA (m) Lentiviral Particles - sc-29809-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU064331 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN27639 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND27639 from Sigma-Aldrich
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BLM siRNA (Mouse) - CRM0387 from Cohesion Biosciences
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BLM - MBS8200007 from MyBioSource
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shRNA set against Mouse BLM (NM_007550.4) - SHH246870 from Creative biogene
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BLM shRNA Plasmid (Mouse) - CHM0387 from Cohesion Biosciences
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