GPAM

Gene Symbol GPAM
Entrez Gene 57678
Alt Symbol GPAT, GPAT1
Species Human
Gene Type protein-coding
Description glycerol-3-phosphate acyltransferase, mitochondrial
Other Description GPAT-1|glycerol 3-phosphate acyltransferase, mitochondrial|glycerol-3-phosphate acyltransferase 1, mitochondrial
Swissprots Q5VW51 Q9HCL2 Q86TA3
Accessions EAW49543 EAW49544 EAW49545 Q9HCL2 AB046780 BAB13386 AK025752 AK172782 BAD18763 AK172799 BAD18772 AL598680 AL832464 AL833061 CAD89967 AL833093 CAD89932 AM392613 CAL37491 BC030783 AAH30783 BQ694715 BX647451 DA313962 DA728196 DA802170 DB197687 XM_005269998 XP_005270055 NM_001244949 NP_001231878 NM_020918 NP_065969
Function Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. {ECO:0000269|PubMed:18238778}.
Subcellular Location Mitochondrion outer membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
Top Pathways Glycerolipid metabolism, Glycerophospholipid metabolism

GPAM (D-10) - sc-398135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GPAM (D-10): sc-398135. Western blot analysis of GPAM expression in human liver tissue extract.

GPAM (H-88) - sc-382257 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

GPAM (L-16) - sc-161674 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-GPAM antibody - ab69990 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 :  Anti-GPAM antibody (ab69990) at 1 µg/mlLane 2 :  Anti-GPAM antibody (ab69990) at 2 µg/mlLane 1 : rat brain tissue lysateLane 2 : rat brain tissue lysateLysates/proteins at 15 µg per lane.

Anti-GPAM antibody - ab107506 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab107506 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107506 at 1 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GPAM antibody - ab68925 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-GPAM antibody (ab68925) at 1/500 dilutionLane 1 : GPAM transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-GPAM Antibody (C-Terminus) IHC-plus™ - LS-B2734 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GPAM antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2734 concentration 5 ug/ml.

Anti-GPAM Antibody (aa426-455, APC) - LS-C235867 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa426-455, AP) - LS-C235866 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa426-455, Biotin) - LS-C235869 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa426-455, FITC) - LS-C235870 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa426-455, PE) - LS-C235874 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa426-455, Azide-free, HRP) - LS-C249107 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody - LS-C307739 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-GPAM Antibody - LS-C311182 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa433-463) - LS-C321715 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPAM Antibody (aa275-325) - LS-C290927 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human GPAM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GPAM antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-GPAM Antibody - LS-C334827 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using GPAM antibody.

Anti-GPAM Antibody (aa426-455) - LS-C166594 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
GPAM Antibody western blot of HepG2 cell line lysates (35 ug/lane). The GPAM antibody detected the GPAM protein (arrow).

GPAM Antibody - NBP1-76907 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: GPAM Antibody [NBP1-76907] - Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 ug/mL.

GPAM Antibody - H00057678-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

GPAM Antibody - NBP2-32116 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GPAM Antibody [NBP2-32116] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells.  Antibodies:  Affinity purified rabbit anti-GPAM antibody NBP2-32116 used for WB at 1 ug/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds.

GPAM Antibody - NBP2-14065 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: GPAM Antibody [NBP2-14065] Staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.

GPAM Antibody - H00057678-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: GPAM Antibody [H00057678-B01P] - Analysis of GPAM expression in transfected 293T cell line by GPAM polyclonal antibody.  Lane 1: GPAM transfected lysate(91.08 KDa). Lane 2: Non-transfected lysate.

GPAM Antibody - H00057678-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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