| Gene Symbol | Fen1 |
|---|---|
| Entrez Gene | 36887 |
| Alt Symbol | CG8648, DmFEN-1, Dmel\CG8648, EG:EG0003.3, FEN1, fen1 |
| Species | Drosophila |
| Gene Type | protein-coding |
| Description | Flap endonuclease 1 |
| Other Description | CG8648-PA|Fen1-PA|flap endonuclease 1|flap endonuclease-1 |
| Swissprots | Q7K7A9 |
| Accessions | AAF57944 CAA21320 O96830 Q7K7A9 BT100031 ACX54939 NM_079041 NP_523765 |
| Function | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {ECO:0000255|HAMAP-Rule:MF_03140}. |
| Subcellular Location | Nucleus, nucleolus {ECO:0000255|HAMAP- Rule:MF_03140}. Nucleus, nucleoplasm {ECO:0000255|HAMAP- Rule:MF_03140}. Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03140}. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. {ECO:0000255|HAMAP-Rule:MF_03140}. |
| Top Pathways | DNA replication, Base excision repair, Non-homologous end-joining |