PDLIM1

Gene Symbol PDLIM1
Entrez Gene 9124
Alt Symbol CLIM1, CLP-36, CLP36, HEL-S-112, hCLIM1
Species Human
Gene Type protein-coding
Description PDZ and LIM domain 1
Other Description LIM domain protein CLP-36|PDZ and LIM domain protein 1|carboxyl terminal LIM domain protein 1|elfin|epididymis secretory protein Li 112
Swissprots Q5VZH5 Q9BPZ9 B2RBS6 O00151
Accessions AAW82438 EAW50009 EAW50010 EAW50011 EAW50012 O00151 AJ310549 CAC32846 AK091847 AK314792 BAG37323 BC000915 AAH00915 BC018755 AAH18755 CD368928 EU446864 ABZ92393 FJ224331 ACI46023 HY025482 U90878 AAC05580 XM_011540330 XP_011538632 NM_020992 NP_066272
Function Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Subcellular Location Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Associates with the actin stress fibers. {ECO:0000250}.
Tissue Specificity Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

CLP36 (B-9) - sc-393084 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CLP36 (B-9): sc-393084. Western blot analysis of CLP36 expression in NIH/3T3 whole cell lysate.

CLP36 (H-8) - sc-398899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CLP36 (H-8): sc-398899. Western blot analysis of CLP36 expression in non-transfected 293T: sc-117752 (A), mouse CLP36 transfected 293T: sc-119315 (B) and NIH/3T3 (C) whole cell lysates.

CLP36 (H-11) - sc-374077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CLP36 (H-11): sc-374077. Western blot analysis of CLP36 expression in Jurkat whole cell lysate.

CLP36 (H-137) - sc-98686 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CLP36 (H-137): sc-98686. Western blot analysis of CLP36 expression in L6 whole cell lysate.

CLP36 (A-6) - sc-365143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CLP36 (A-6): sc-365143. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

CLP36 (V-14) - sc-79911 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CLP36 (V-14): sc-79911. Western blot analysis of CLP36 expression in non-transfected 293T: sc-117752 (A), mouse CLP36 transfected 293T: sc-119315 (B) and HeLa (C) whole cell lysates.

CLP36 (N-14) - sc-79909 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CLP36 (N-14): sc-79909. Western blot analysis of CLP36 expression in HeLa whole cell lysate.

CLP36 (51) - sc-136420 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CLP36 (51): sc-136420. Western blot analysis of CLP36 expression in NRK whole cell lysate.

Anti-PDLIM1 antibody - ab64971 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
PDLIM1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to PDLIM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64971.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.Band: 36kDa: PDLIM1.

Anti-PDLIM1 antibody - ab199626 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PDLIM1 antibody (ab199626) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

Anti-PDLIM1 antibody - ab115842 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Human Placenta (formalin-fixed, paraffin-embedded) stained with ab115842 at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PDLIM1 antibody - ab133711 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labelling PDLIM1 with ab133711 at 15 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PDLIM1 antibody - ab17022 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PDLIM1 antibody (ab17022) at 0.1 µg/ml + Human lung lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-PDLIM1 antibody [EPR7186(B)] - ab129015 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7186(B)
All lanes : Anti-PDLIM1 antibody [EPR7186(B)] (ab129015) at 1/1000 dilutionLane 1 : Human heart cell lysateLane 2 : L6 cell lysateLane 3 : 293T cell lysateLane 4 : Caco 2 cell lysateLane 5 : Saos 2 cell lysateLane 6 : C2C12 cell lysateLane 7 : Rat lung cell lysateLane 8 : Mouse lung cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Anti-PDLIM1 antibody [EPR7187] - ab126628 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7187
All lanes : Anti-PDLIM1 antibody [EPR7187] (ab126628) at 1/1000 dilutionLane 1 : Jurkat cell line lysateLane 2 : HeLa cell line lysateLane 3 : 293T cell line lysateLane 4 : Caco 2 cell line lysateLane 5 : Saos 2 cell line lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-PDLIM1 Antibody (aa316-329) IHC-plus™ - LS-B2418 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-PDLIM1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2418 concentration 5 ug/ml.

Anti-PDLIM1 Antibody (Internal, clone EPR7186(B)) IHC-plus™ - LS-B11094 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7186(B)
Anti-PDLIM1 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11094 dilution 1:100.

Anti-PDLIM1 Antibody - LS-C334876 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using PDLIM1 antibody.

Anti-PDLIM1 Antibody (aa254-282, APC) - LS-C216677 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDLIM1 Antibody (aa165-214) - LS-C30780 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
NIH 3T3 Cell Lysate

Anti-PDLIM1 Antibody (aa254-282, AP) - LS-C216678 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDLIM1 Antibody (clone 2D4) - LS-C133993 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D4
PDLIM1 monoclonal antibody (M02), clone 2D4. Western blot of PDLIM1 expression in Jurkat.

Anti-PDLIM1 Antibody (aa254-282, Biotin) - LS-C216679 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDLIM1 Antibody (aa254-282, FITC) - LS-C216680 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PDLIM1 Antibody (aa254-282, Azide-free, HRP) - LS-C216681 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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