ARAP1

Gene Symbol ARAP1
Entrez Gene 116985
Alt Symbol CENTD2
Species Human
Gene Type protein-coding
Description ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Other Description ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1|arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1|centaurin, delta 2|centaurin-delta-2|cnt-d2
Swissprots O94879 Q59FI7 Q96L71 Q8WU51 A3KLL7 Q6PHS3 B2RTS2 Q4LDD5 Q96HP6 Q96P48
Accessions EAW74863 EAW74864 EAW74865 EAW74866 Q96P48 AB018325 BAA34502 AF411983 AAL04167 AJ621557 CAF21317 AJ706973 AK160383 BAD18726 AY049732 AAL12169 AY553630 AAT36325 BC008315 AAH08315 BC021244 BC056401 AAH56401 BC140792 AAI40793 BC144562 AAI44563 BQ921498 CD251362 DA186424 NM_001040118 NP_001035207 NM_001135190 NP_001128662 NM_015242 NP_056057
Function Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell membrane. Note=Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
Tissue Specificity Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea. {ECO:0000269|PubMed:11804590}.
Top Pathways Endocytosis

ARAP1 (A-3) - sc-393138 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARAP1 (A-3): sc-393138. Western blot analysis of ARAP1 expression in K-562 (A), HeLa (B) and NIH/3T3 (C) whole cell lysates.

ARAP1 (H-120) - sc-367892 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ARAP1 (H-120): sc-367892. Western blot analysis of ARAP1 expression in NIH/3T3 whole cell lysate.

ARAP1 (C-16) - sc-47796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ARAP1 (N-20) - sc-47799 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARAP1 (N-20): sc-47799. Western blot analysis of ARAP1 expression in K-562 whole cell lysate.

Anti-ARAP1 antibody - ab5912 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab5912 staining ARAP1 in murine NIH3T3 cells by Immunocytochemistry/ Immunofluorescence.

Anti-ARAP1 antibody - ab199180 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling ARAP1 using ab199180 at 1/40 dilution.

Anti-ARAP1 antibody - ab99382 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARAP1 antibody (ab99382) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Anti-ARAP1 antibody - ab96823 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-ARAP1 antibody (ab96823) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-ARAP1 antibody [ARAP1-2] - ab64605 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ARAP1-2
ICC/IF image of ab64605 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64605, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CENTD2 / ARAP1 Antibody (aa1160-1210) - LS-C288209 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CENTD2 / ARAP1 Antibody (aa1388-1450) - LS-C185785 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate). A: H1299. 5% SDS PAGE. CENTD2 / ARAP1 antibody diluted at 1:1000.

Anti-CENTD2 / ARAP1 Antibody (C-Terminus) - LS-C54490 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CENTD2 / ARAP1 Antibody (aa1421-1436) - LS-C148977 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CENTD2 / ARAP1 Antibody (aa1086-1103) - LS-C149004 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

ARAP1 Antibody - NB100-68223 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARAP1 Antibody [NB100-68223] - Detection of Human CENTD2/ARAP1 on HeLa whole cell lysate using NB100-68223. CENTD2/ARAP1 was also immunoprecipitated by another rabbit anti-CENTD2/ARAP1 antibody.

ARAP1 Antibody (ARAP1-2) - NB110-68801 from Novus Biologicals

Prices $279.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ARAP1-2
Immunocytochemistry/Immunofluorescence: ARAP1 Antibody (ARAP1-2) [NB110-68801] - Colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0, 30 or 60 and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.

ARAP1 Antibody - H00116985-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot analysis of CENTD2 expression in transfected 293T cell line by CENTD2 MaxPab polyclonal antibody. Lane 1: CENTD2 transfected lysate (132.55 KDa). Lane 2: Non-transfected lysate.

ARAP1 Antibody - 31230002 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ARAP1 Antibody (ARAP1-2) [Allophycocyanin] - NB110-68801APC from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2

ARAP1 Antibody (ARAP1-2) [DyLight 350] - NB110-68801UV from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2

ARAP1 Antibody (ARAP1-2) [DyLight 488] - NB110-68801G from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2

ARAP1 Antibody (ARAP1-2) [DyLight 405] - NB110-68801V from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2

ARAP1 Antibody (ARAP1-2) [PE] - NB110-68801PE from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2

ARAP1 Antibody (ARAP1-2) [PerCP] - NB110-68801PCP from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2

ARAP1 Antibody (ARAP1-2) [7C] - NB110-68801V2 from Novus Biologicals

Host Mouse
Clonality Monoclonal
Clone ARAP1-2
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