MRRF

Gene Symbol	MRRF
Entrez Gene	92399
Alt Symbol	MRFF, MTRRF, RRF
Species	Human
Gene Type	protein-coding
Description	mitochondrial ribosome recycling factor
Other Description	ribosome-recycling factor, mitochondrial\|ribosome-releasing factor, mitochondrial
Swissprots	B7Z4X5 Q5T7T2 Q5T7T0 Q5T7T1 B7Z6P7 Q5T7T3 Q5T7T5 A8K6D8 Q5T7T4 A8K6Z4 Q96E11 Q5RKT1
Accessions	EAW87520 EAW87521 EAW87522 EAW87523 EAW87524 EAW87525 EAW87526 EAW87527 EAW87528 Q96E11 AK291603 BAF84292 AK291809 BAF84498 AK292790 BAF85479 AK298046 BAH12711 AK300718 BAH13333 AK310974 AK316266 BAH14637 BC002814 BC013049 AAH13049 BC053604 AAH53604 BF982227 HG502399 HQ447351 ADQ31837 XM_005252302 XP_005252359 XM_011519183 XP_011517485 XM_011519184 XP_011517486 XM_011519185 XP_011517487 XM_011519186 XP_011517488 XM_011519187 XP_011517489 XR_242607 XR_428543 NM_199176 NM_001173512 NP_001166983 NM_138777 NP_620132 NM_199177 NP_954646
Function	Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another (By similarity). {ECO:0000250}.
Subcellular Location	Mitochondrion {ECO:0000250}.

MRRF (D-17) - sc-165033 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

MRRF (E-14) - sc-165034 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

MRRF (E-14): sc-165034. Western blot analysis of MRRF expression in A-431 (A) and HeLa (B) whole cell lysates, HeLa nuclear extract (C) and mouse lung tissue extract (D).

MRRF (J-Q7) - sc-100969 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/1.0 ml
Host	Mouse
Clonality	Monoclonal

MRRF (J-Q7): sc-100969. Western blot analysis of MRRF expression in non-transfected 293: sc-110760 (A), human MRRF transfected 293: sc-113108 (B) and A-431 (C) whole cell lysates.

Anti-MRRF antibody - ab92659 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-MRRF antibody (ab92659) at 1/500 dilutionLane 1 : HeLa cell lysate in absence of blocking peptideLane 2 : HeLa cell lysate in presence of blocking peptide (10 &microg)Lysates/proteins at 30 µg per lane.

Anti-MRRF antibody - ab199674 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-MRRF antibody (ab199674) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

Anti-MRRF antibody - ab57943 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

MRRF antibody (ab57943) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.

Anti-MRRF antibody - ab88961 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Anti-MRRF antibody (ab88961) at 1/500 dilution + human colon tissue lysate at 50 µg

Anti-MRRF antibody [EPR14140(B)] - ab181223 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR14140(B)

All lanes : Anti-MRRF antibody [EPR14140(B)] (ab181223) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLane 3 : A431 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-MRRF Antibody - LS-C311414 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-MRRF Antibody (clone AT7D10) - LS-C175822 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	AT7D10

Western Blot: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRRF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-MRRF Antibody (aa56-262, clone 7F1) - LS-C339829 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	7F1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRRF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRRF.

Anti-MRRF Antibody (aa181-230) - LS-C120342 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from HeLa cells, using MRRF Antibody. The lane on the right is treated with the synthesized peptide.

Anti-MRRF Antibody (aa56-262, clone 1B8) - LS-C339878 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1B8

Anti-MRRF Antibody (aa56-262, clone 2A9) - LS-C339837 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2A9

IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MRRF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MRRF Antibody (aa56-262, clone 2A8) - LS-C339917 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2A8

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRRF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MRRF Antibody (clone 1D3) - LS-C133827 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1D3

Immunoperoxidase of monoclonal antibody to MRRF on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Anti-MRRF Antibody (aa56-262, clone 1D5) - LS-C339798 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1D5

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-MRRF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

MRRF Antibody (1D3) - H00092399-M01 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1D3

Western Blot: MRRF Antibody (1D3) [H00092399-M01] - Western Blot analysis of MRRF expression in A-431 ( Cat # L015V1 ).

MRRF Antibody - NBP2-33481 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: MRRF Antibody [NBP2-33481] - liver

MRRF Antibody - NBP2-15025 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: MRRF Antibody [NBP2-15025] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 12% SDS PAGE, antibody diluted at 1:2000.

MRRF Antibody - H00092399-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: MRRF Antibody [H00092399-B01] - MRRF MaxPab polyclonal antibody (B01), Lot # 08046 WULz. Western Blot analysis of MRRF expression in human colon.

MRRF Antibody - NBP2-33586 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: MRRF Antibody [NBP2-33586] - Immunofluorescent staining of human cell line HEK 293 shows localization to microtubules & cytokinetic bridge.

MRRF Antibody (7D10) - NBP2-22564 from Novus Biologicals

Prices	$279.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	7D10

MRRF Antibody - H00092399-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: MRRF Antibody [H00092399-D01P] - analysis of MRRF expression in transfected 293T cell line by MRRF MaxPab rabbit polyclonal antibody. Lane 1: MRRF transfected lysate (29.30 KDa). Lane 2: Non-transfected lysate.

MRRF Antibody (3E3) - H00092399-M11 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3E3

Western Blot: MRRF Antibody (3E3) [H00092399-M11] - Analysis of MRRF expression in transfected 293T cell line by MRRF monoclonal antibody (M11), clone 3E3. Lane 1: MRRF transfected lysatE (29.3 KDa). Lane 2: Non-transfected lysate.

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