TOP3A

Gene Symbol TOP3A
Entrez Gene 7156
Alt Symbol TOP3, ZGRF7
Species Human
Gene Type protein-coding
Description topoisomerase (DNA) III alpha
Other Description DNA topoisomerase 3-alpha|DNA topoisomerase III alpha|topo III-alpha|zinc finger, GRF-type containing 7
Swissprots Q13472 Q13473 A8KA61 D3DXC7 B4DK80
Accessions ADP90454 ADP90455 ADP90456 ADP90457 ADP90458 ADP90459 ADP90460 ADP90461 ADP90462 ADP90463 ADP90464 ADP90465 ADP90466 ADP90467 ADP90468 ADP90469 ADP90470 ADP90471 ADP90472 ADP90473 ADP90474 ADP90475 ADP90476 ADP90477 ADP90478 ADP90479 ADP90480 ADP90481 ADP90482 ADP90483 ADP90484 ADP90485 ADP90486 ADP90487 ADP90488 ADP90489 ADP90490 ADP90491 ADP90492 ADP90493 EAW55646 EAW55647 EAW55648 EAW55649 Q13472 AF086042 AK126869 BAG54385 AK290513 BAF83202 AK292926 BAF85615 AK296437 BAG59092 AK299764 BAG61652 AK311668 BC051748 AAH51748 BM462184 U43431 AAB03694 XM_005256776 XP_005256833 XM_011524000 XP_011522302 XM_011524001 XP_011522303 NM_004618 NP_004609
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. {ECO:0000269|PubMed:20445207, ECO:0000269|PubMed:8622991}.
Tissue Specificity High expression is found in testis, heart, skeletal muscle and pancreas.
Top Pathways Homologous recombination, Fanconi anemia pathway

Topo IIIα (N-20) - sc-11257 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Topo IIIα (N-20): sc-11257. Western blot analysis of Topo IIIα expression in HeLa (A) and K-562 (B) nuclear extracts.

Topo IIIα (H-300) - sc-13060 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Topo IIIα (E-17) - sc-11258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Topoisomerase III alpha antibody - ab108493 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/mlLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Topoisomerase III alpha antibody [1A4] - ChIP Grade - ab49673 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1A4

TOP3A Antibody (OAAF02411) - OAAF02411 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

TOP3A Polyclonal Antibody - A4163 from ABclonal

Prices $200.00, $480.00
Sizes 50 µg, 200 µg
Host Rabbit
Clonality Polyclonal

TOP3A antibody - 70R-20925 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

TOP3A Antibody - abx003076 from Abbexa

Host Rabbit
Clonality Polyclonal

Topoisomerase 3 alpha Antibody - abx121230 from Abbexa

Host Rabbit
Clonality Polyclonal

TOP3A antibody - 14525-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
K562 cell were subjected to SDS PAGE followed by western blot with 14525-1-AP(TOP3A Antibody) at dilution of 1:1200

Anti-TOP3A, Polyclonal Antibody - 25-A4163 from ARP American Research Products

Prices $299.00, $449.00
Sizes 100 µg, 200 µg
Host Rabbit
Clonality Polyclonal

TOP3A antibody - STJ25923 from St John's Laboratory Ltd

Host Rabbit
Clonality Polyclonal

TOP3A antibody - 14525-1-AP from Proteintech Group

Prices $279.00
Sizes con: 65 μg/150μl
Host Rabbit
Clonality Polyclonal
K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP(TOP3A antibody) at dilution of 1:1000

TOP3A - MBS129665 from MyBioSource

Prices $285.00, $555.00
Sizes 50 µg, 200 µg
Host Rabbit
Clonality Polyclonal

topoisomerase (DNA) III alpha , Polyclonal Antibody - MBS712849 from MyBioSource

Prices $230.00, $500.00
Sizes 50 µl, 150 µl
Host Rabbit
Clonality Polyclonal

TOP3A - A4163 from NeoScientific

Prices $480.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

TOP3A Antibody - E11-10982C from Enogene

Prices $225.00
Sizes 100 μg
Host Rabbit

Monoclonal Antibody to Topoisomerase III (TOP3) - MAD800Hu21 from Uscn Life Science Inc.

Host Mouse
Clonality Monoclonal

Polyclonal Antibody to Topoisomerase III (TOP3) - PAD800Hu01 from Uscn Life Science Inc.

Host Rabbit
Clonality Polyclonal

Anti-TOP3A (aa 900-1000) polyclonal antibody - DPABH-25482 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-Topoisomerase 3 alpha Antibody - CPA3729 from Cohesion Biosciences

Prices $110.00, $220.00, $350.00
Sizes 30 µl, 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of Topoisomerase 3 alpha expression in A549 (A); MCF7 (B) whole cell lysates.

TOP3A polyclonal antibody - BS60749 from Bioworld Technology

Prices $158.00, $275.00
Sizes 50 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western blot (WB) analysis of TOP3A pAb at 1:500 dilution Lane1:A549 whole cell lysate Lane2:MCF-7 whole cell lysate

TOP3A Antibody - A02T0175 from BlueGene Biotech

Monoclonal Antibody to Topoisomerase III (TOP3) - MAD800Hu21 from Cloud-clone

Host Mouse
Clonality Monoclonal
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