MID2

Gene Symbol MID2
Entrez Gene 11043
Alt Symbol FXY2, MRX101, RNF60, TRIM1
Species Human
Gene Type protein-coding
Description midline 2
Other Description RING finger protein 60|midin 2|midin-2|midline defect 2|midline-2|probable E3 ubiquitin-protein ligase MID2|tripartite motif protein 1|tripartite motif-containing protein 1
Swissprots Q5JYF5 Q9UJR9 A6PVI5 A6NEL8 Q9UJV3 Q8WWK1
Accessions EAX02701 EAX02702 EAX02703 Q9UJV3 AF196481 AAF07341 AK095034 AK123807 AY625004 AAT48105 BC017707 AAH17707 BC035557 BC058011 BC070370 BT006663 AAP35309 DN997921 JF432292 ADZ15509 Y18880 CAB56154 XM_005262062 XP_005262119 NM_012216 NP_036348 NM_052817 NP_438112
Function May play a role in microtubule stabilization. {ECO:0000303|PubMed:24115387}.
Subcellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
Tissue Specificity Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.

Midline-2 (K-15) - sc-55130 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Midline-2 (K-15): sc-55130. Western blot analysis of Midline-2 expression in non-transfected: sc-117752 (A) and human Midline-2 transfected: sc-158731 (B) 293T whole cell lysates.

Midline-2 (E-19) - sc-55129 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MID2 antibody - ab14749 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-MID2 antibody (ab14749) at 1 µg/ml + Mouse Heart lysate (RIPA buffer, 35µg total protein per lane).

Anti-MID2 antibody - ab168915 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-MID2 antibody (ab168915) at 1 µg/mlLane 1 : MID2 recombinant fragment transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µg per lane.

Anti-TRIM1 / MID2 Antibody (aa690-717) - LS-C164193 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MID2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human pancreas tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-TRIM1 / MID2 Antibody (aa723-735) IHC-plus™ - LS-B2277 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-MID2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2277 concentration 5 ug/ml.

Anti-TRIM1 / MID2 Antibody (aa690-717, AP) - LS-C237638 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa690-717, APC) - LS-C237639 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa690-717, Biotin) - LS-C237641 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa690-717, FITC) - LS-C237640 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa690-717, PE) - LS-C237644 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa690-717, Azide-free, HRP) - LS-C248818 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody - LS-C307609 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa669-698) - LS-C323004 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TRIM1 / MID2 Antibody (aa210-259) - LS-C134990 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MID2 antibody LS-C134990 Western blot of Small Intestine lysate. Antibody concentration 1 ug/ml.

Anti-TRIM1 / MID2 Antibody (aa455-504) - LS-C201711 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human NCI-H226. MID2 antibody dilution 1.0 ug/ml.

MID2 Antibody - NB100-1167 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: MID2 Antibody [NB100-1167] - Analysis of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35ug protein in RIPA buffer).

MID2 Antibody - NBP1-81862 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MID2 Antibody [NBP1-81862] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissue

MID2 Antibody - NBP2-17288 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MID2 Antibody [NBP2-17288] - Western Blot analysis of MID2 expression in transfected 293T cell line by MID2 polyclonal antibody. A: Non-transfected lysate. B: MID2 transfected lysate. 12% SDS PAGE gel, diluted at 1:5000.

MID2 Antibody - H00011043-B02P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

MID2 Antibody - H00011043-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: MID2 Antibody [H00011043-B01] - analysis of MID2 expression in transfected 293T cell line by MID2 MaxPab polyclonal antibody (B01). Lane 1: MID2 transfected lysate (75.35 KDa). Lane 2: Non-transfected lysate.

MID2 Antibody - H00011043-B02 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: MID2 Antibody [H00011043-B02] - analysis of MID2 expression in transfected 293T cell line by MID2 MaxPab polyclonal antibody (B02). Lane 1: MID2 transfected lysate (77.90 KDa). Lane 2: Non-transfected lysate.

Anti-MID2/TRIM1 antibody produced in goat - SAB2500634 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MID2 / TRIM1: <B>Cat. No. SAB2500634</B>: (1 μg/mL) staining of mouse heart lysate (RIPA buffer, 35 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-MID2 (121-133) antibody produced in rabbit - M2448 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MID2 (121-133) (RB): <B>Cat. No. M2448</B>: Immunofluorescence of HUVEC cells using MID2 (121-133) (RB), <B>Cat. No. M2448</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.<BR/>Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-MID2 (631-645) antibody produced in rabbit - M2573 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MID2 (631-645): <B>Cat. No. M2573</B>: Immunofluorescence of HUVEC cells using MID2 (631-645) (RB),<B>Cat. No. M2573</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
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