CYP7B1

Gene Symbol CYP7B1
Entrez Gene 9420
Alt Symbol CBAS3, CP7B, SPG5A
Species Human
Gene Type protein-coding
Description cytochrome P450, family 7, subfamily B, polypeptide 1
Other Description 25-hydroxycholesterol 7-alpha-hydroxylase|cytochrome P450 7B1|cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1|oxysterol 7-alpha-hydroxylase|oxysterol 7alpha-hydroxylase
Swissprots O75881 Q9UNF5 B2RN07
Accessions AAK11850 EAW86877 O75881 AF029403 AAC95426 AF127090 AAD20021 BC029155 AAH29155 BC136574 AAI36575 NM_004820 NP_004811
Subcellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue Specificity Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
Top Pathways Primary bile acid biosynthesis, Steroid hormone biosynthesis

CYP7B1 (WW-H9) - sc-134309 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
CYP7B1 (WW-H9): sc-134309. Western blot analysis of CYP7B1 expression in human CYP7B1 transfected (A) and non-transfected (B) 293T whole cell lysates.

CYP7B1 (H-300) - sc-367167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

CYP7B1 (N-17) - sc-26087 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CYP7B1 (N-17): sc-26087. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.

Anti-CYP7B1 antibody - ab175889 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CYP7B1 antibody (ab175889) at 1/1000 dilution + HeLa cell lysate at 35 µg

Anti-CYP7B1 antibody - ab136801 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of CYP7B1 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136801, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CYP7B1 antibody - ab19043 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab19043 at 4µg/ml, staining CYP7B1 in Human Breast tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Image shows textured cytoplasm staining in ductal epithelial cells. Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.

Anti-CYP7B1 antibody - ab77157 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-CYP7B1 antibody (ab77157) at 5 µg/ml + Recombinant tagged human CYP7B1 fragment at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-CYP7B1 antibody - ab74858 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CYP7B1 antibody (ab74858) at 1/500 dilutionLane 1 : extracts from LOVO cellsLane 2 : extracts from LOVO cells with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-CYP7B1 antibody - ab172340 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-CYP7B1 antibody (ab172340) at 1 µg/mlLane 1 : CYP7B1 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-CYP7B1 antibody [EPR8395] - ab138497 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8395
All lanes : Anti-CYP7B1 antibody [EPR8395] (ab138497) at 1/1000 dilutionLane 1 : PC3 lysateLane 2 : Human fetal liver lysateLane 3 : Human fetal kidney lysateLysates/proteins at 30 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-CYP7B1 antibody - C-terminal - ab155923 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CYP7B1 antibody - C-terminal (ab155923) at 1/1500 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-CYP7B1 Antibody (C-Terminus) - LS-C54696 from LifeSpan Bioscience

Host Goat
Clonality Polyclonal
CYP7B1 antibody (4 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-CYP7B1 Antibody (clone 7H10) - LS-C114413 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 7H10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY cyp7b1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-cyp7b1.

Anti-CYP7B1 Antibody (aa101-150) - LS-C119512 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot of extracts from LOVO cells, using Cytochrome P450 7B1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-CYP7B1 Antibody (aa304-353) - LS-C135895 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CYP7B1 antibody LS-C135895 Western blot of 721_B Cell lysate. Antibody concentration 1 ug/ml.

Anti-CYP7B1 Antibody (Gln127) - LS-C177344 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
IHC of CYP7B1 (Q127) pAb in paraffin-embedded human skeletal muscle tissue.

Anti-CYP7B1 Antibody (clone 7E5) - LS-C114345 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 7E5
Cyp7b1 antibody (7E5) at 1:500 dilution + lysates from HEK-293T transfected with human Cyp7b1 expression vector .

Anti-CYP7B1 Antibody (clone 2B11) - LS-C134000 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 2B11
Immunoperoxidase of monoclonal antibody to CYP7B1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]

Anti-CYP7B1 Antibody (aa259-289) - LS-C167245 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human brain tissue reacted with CYP7B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

CYP7B1 Antibody - NBP2-31092 from Novus Biologicals

Prices $149.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

CYP7B1 Antibody (2B11) - H00009420-M06 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B11
Western Blot: CYP7B1 Antibody (2B11) [H00009420-M06] - Analysis of CYP7B1 expression in transfected 293T cell line by CYP7B1 monoclonal antibody (M06), clone 2B11. Lane 1: CYP7B1 transfected lysatE (58.256 KDa). Lane 2: Non-transfected lysate.

CYP7B1 Antibody - NBP1-00774 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: CYP7B1 Antibody [NBP1-00774] - Western blot (WB) analysis of CYP7B1 (Q127) antibody in extracts from LOVO cells

CYP7B1 Antibody - H00009420-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: CYP7B1 Antibody [H00009420-A01] - Analysis of CYP7B1 expression in NIH/3T3 (Cat # L018V1).

CYP7B1 Antibody (7E5) - NBP1-47722 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7E5
Western Blot: CYP7B1 Antibody (7E5) [NBP1-47722] Analysis of extracts (35ug) from 9 different cell lines by using anti-CYP7B1 monoclonal antibody.

CYP7B1 Antibody - NBP1-87013 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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