DNAJB1

Gene Symbol DNAJB1
Entrez Gene 3337
Alt Symbol HSPF1, Hdj1, Hsp40, RSPH16B, Sis1
Species Human
Gene Type protein-coding
Description DnaJ (Hsp40) homolog, subfamily B, member 1
Other Description dnaJ homolog subfamily B member 1|dnaJ protein homolog 1|heat shock 40 kDa protein 1|human DnaJ protein 1|radial spoke 16 homolog B
Swissprots P25685 B4DX52
Accessions BAA12819 CCD35253 CEO43497 EAW84432 EAW84433 P25685 AK092989 AK296609 BAG59221 AK301817 BAG63264 AK312624 BAG35510 BC002352 AAH02352 BC013157 BC019827 AAH19827 BM795601 CR536485 CAG38724 CR541677 CAG46478 D49547 BAA08495 DA377896 DB454482 HY032238 HY042250 X62421 CAA44287 XM_006722733 XP_006722796 XM_006722734 XP_006722797 XM_011527956 XP_011526258 XM_011527957 XP_011526259 XM_011527958 XP_011526260 NM_001300914 NP_001287843 NM_006145 NP_006136
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:1586970}. Nucleus {ECO:0000269|PubMed:1586970}. Nucleus, nucleolus {ECO:0000269|PubMed:1586970}. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Top Pathways Protein processing in endoplasmic reticulum, Influenza A

HSP40 (C64B4) Rabbit mAb - 4871 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C64B4
Western blot analysis of extracts from HeLa, C2C12, C6 and COS cells using HSP40 (C64B4) Rabbit mAb.

HSP40 Antibody - 4868 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, C2C12 and C6 cells, using HSP40 Antibody.

HSP 40 (B-3) - sc-398766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSP 40 (B-3): sc-398766. Western blot analysis of HSP 40 expression in Jurkat (A) and K-562 (B) whole cell lysates.

HSP 40 (C-20) - sc-1800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSP 40 (C-20): sc-1800. Immunofluorescence staining of methanol-fixed HeLa cells showing localization of HSP 40.

HSP 40 (G-12) - sc-49988 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HSP 40 (H-100) - sc-28845 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HSP 40 (H-100): sc-28845. Western blot analysis ofHSP 40 expression in non-transfected: sc-117752 (A) and human HSP 40 transfected: sc-114001 (B) 293T whole cell lysates.

HSP 40 (N-19) - sc-1801 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HSP 40 (5) - sc-135943 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
HSP 40 (5): sc-135943. Western blot analysis of HSP 40 expression in HeLa whole cell lysate.

Anti-Hsp40 antibody - ab69402 from Abcam

Prices $384.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hsp40 antibody (ab69402) at 1/1000 dilutionLane 1 : Molecular weight markerLane 2 : Hsp40 recombinant proteinLane 3 : DnaJ recombinant proteinLane 4 : Cell lysates prepared from heat shocked hela cellsLane 5 : Cell lysates prepared from heat shocked 3T3 cellsLane 6 : Cell lysates prepared from PC-12 cells

Anti-Hsp40 antibody - ab110631 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistological staining of Human Placenta tissue, Formalin-Fixed and Paraffin-Embedded (FFPE) using ab110631 at 10µg/ml dilution.

Anti-Hsp40 antibody - ab53108 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hsp40 antibody (ab53108) at 1/300 dilutionLane 1 : COLO205 cell extractLane 2 : COLO205 cell extract with immunising peptide

Anti-Hsp40 antibody - ab49994 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Hsp40 antibody [1H8] - ab117958 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H8
All lanes : Anti-Hsp40 antibody [1H8] (ab117958) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Hsp40 cDNALysates/proteins at 5 µg per lane.

Anti-Hsp40 antibody [3B9.E6] - ab78437 from Abcam

Prices $381.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B9.E6
Overlay histogram showing HeLa cells stained with ab78437 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78437, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hsp40 antibody [k1C7] - ab58978 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone k1C7
Anti-Hsp40 antibody [k1C7] (ab58978) at 1/1000 dilution + Jurkat cell lysate at 50 µgSecondaryHRP-conjugated goat anti-mousedeveloped using the ECL technique

Anti-DNAJB1 / Hsp40 Antibody (aa1-340) - LS-C36173 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DNAJB1 / Hsp40 Antibody - LS-C36621 from LifeSpan Bioscience

Prices $375.00
Sizes 25 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJB1 / Hsp40 Antibody (aa1-340) - LS-C45029 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-DNAJB1 / Hsp40 Antibody (aa170-220) - LS-C90359 from LifeSpan Bioscience

Prices $295.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-DNAJB1 / Hsp40 Antibody (C-Terminus) - LS-C121937 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DNAJB1 / Hsp40 Antibody - LS-C123722 from LifeSpan Bioscience

Prices $355.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal

Anti-DNAJB1 / Hsp40 Antibody (C-Terminus, Sepharose) - LS-C171757 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DNAJB1 / Hsp40 Antibody (C-Terminus, Magnetic beads) - LS-C171756 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DNAJB1 / Hsp40 Antibody (Internal, clone EPR1530) - LS-C138308 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1530
Western blot analysis on (A) Jurkat, (B) HepG2, (C) HeLa and (D) 293T cell lysates using anti-HSP40 antibody.

Anti-DNAJB1 / Hsp40 Antibody (C-Terminus) - LS-C146350 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DNAJB1 / Hsp40 antibody LS-C146350 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.
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