Gene Symbol | Diap1 |
---|---|
Entrez Gene | 13367 |
Alt Symbol | D18Wsu154e, Dia1, Diaph1, Drf1, p140mDia |
Species | Mouse |
Gene Type | protein-coding |
Description | diaphanous homolog 1 (Drosophila) |
Other Description | diaphanous-related formin-1|protein diaphanous homolog 1 |
Swissprots | O08808 |
Accessions | EDL10115 EDL10116 EDL10117 O08808 AK012707 BAB28425 AK135915 BAE22723 AK140734 BAE24459 AK156391 BAE33697 AK220466 BAD90493 BC012974 BC021396 AAH21396 BC024979 BC029331 BC070412 AAH70412 U96963 AAC53280 XM_006525589 XP_006525652 XM_011246826 XP_011245128 NM_001305980 NP_001292909 NM_001305981 NP_001292910 NM_007858 NP_031884 |
Function | Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. The MEMO1- RHOA-DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stab |
Subcellular Location | Cell membrane {ECO:0000269|PubMed:9214622}. Cell projection, ruffle membrane {ECO:0000269|PubMed:9214622}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:9214622}. Note=Membrane ruffles, especially at the tip of ruffles, of motile cells. |
Tissue Specificity | Ubiquitous. |
Top Pathways | Focal adhesion, Regulation of actin cytoskeleton |
Dia 1 CRISPR/Cas9 KO Plasmid (m) - sc-420000 from Santa Cruz Biotechnology
|
||||||||
Dia 1 HDR Plasmid (m) - sc-420000-HDR from Santa Cruz Biotechnology
|
||||||||
Dia 1 Double Nickase Plasmid (m) - sc-420000-NIC from Santa Cruz Biotechnology
|
||||||||
Dia 1 Double Nickase Plasmid (m2) - sc-420000-NIC-2 from Santa Cruz Biotechnology
|
||||||||
Dia 1 CRISPR Activation Plasmid (m) - sc-420000-ACT from Santa Cruz Biotechnology
|
||||||||
Dia 1 CRISPR Activation Plasmid (m2) - sc-420000-ACT-2 from Santa Cruz Biotechnology
|
||||||||
Dia 1 Lentiviral Activation Particles (m) - sc-420000-LAC from Santa Cruz Biotechnology
|
||||||||
Dia 1 Lentiviral Activation Particles (m2) - sc-420000-LAC-2 from Santa Cruz Biotechnology
|
||||||||
Dia 1 siRNA (m) - sc-35191 from Santa Cruz Biotechnology
|
||||||||
Dia 1 shRNA Plasmid (m) - sc-35191-SH from Santa Cruz Biotechnology
|
||||||||
Dia 1 shRNA (m) Lentiviral Particles - sc-35191-V from Santa Cruz Biotechnology
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN29991 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND29991 from Sigma-Aldrich
|
||||||||
DIAPH1 siRNA (Mouse) - CRM1027 from Cohesion Biosciences
|
||||||||
DIAPH1 - MBS8238670 from MyBioSource
|
||||||||
shRNA set against Mouse Diap1 (NM_007858.2) - SHH278017 from Creative biogene
|
||||||||
DIAPH1 shRNA Plasmid (Mouse) - CHM1027 from Cohesion Biosciences
|
||||||||
DIAP1 miRNA 3'UTR clone - MiUTR1M-03860 from Creative biogene
|
||||||||