Gene Symbol | FAN1 |
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Entrez Gene | 22909 |
Alt Symbol | KIAA1018, KMIN, MTMR15, hFAN1 |
Species | Human |
Gene Type | protein-coding |
Description | FANCD2/FANCI-associated nuclease 1 |
Other Description | coiled-coil domain-containing protein MTMR15|fanconi anemia associated nuclease 1|fanconi-associated nuclease 1|myotubularin-related protein 15 |
Swissprots | Q9Y2M0 Q86WU8 A8K4M2 |
Accessions | EAW61257 Q9Y2M0 AB023235 BAA76862 AK026787 AK290987 BAF83676 BC044890 BC047882 AAH47882 BP310263 BX647391 CR936727 CR936734 DA841207 EU832018 ACE86680 HG506830 HM560957 ADK11100 KJ534972 AHW56612 KJ534973 AHW56613 KJ535046 AHW56685 XM_005254232 XP_005254289 XM_005254234 XP_005254291 XM_005254235 XP_005254292 XM_005254236 XP_005254293 XM_006725538 XP_006725601 XM_006725539 XP_006725602 XM_006725540 XP_006725603 XM_006725541 XP_006725604 XM_011521370 XP_011519672 XM_011521371 XP_011519673 XM_011521372 XP_011519674 XM_011547388 XP_011545690 XM_011547389 XP_011545691 XM_011547390 XP_011545692 XM_011548898 XP_011547200 XM_011548899 XP_011547201 XM_011548900 XP_011547202 XM_011548901 XP_011547203 XM_011548902 XP_011547204 XM_011548903 XP_011547205 XM_011548904 XP_011547206 NM_001146094 NP_001139566 NM_001146095 NP_001139567 NM_001146096 NP_001139568 NM_014967 NP_055782 |
Function | Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771). Also has endonuclease activity toward 5'-flaps (PubMed:206 |
Subcellular Location | Nucleus {ECO:0000269|PubMed:20603015, ECO:0000269|PubMed:20603016, ECO:0000269|PubMed:20603073, ECO:0000269|PubMed:20671156, ECO:0000269|PubMed:20935496}. Note=Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2 (PubMed:20603015, PubMed:20603016). Localizes to stalled replication forks via its UBZ-type zinc finger (PubMed:20935496). {ECO:0000269|PubMed:20603015, ECO:0000269|PubMed:20603016, ECO:0000269|PubMed:20935496}. |
Top Pathways | Fanconi anemia pathway |
MTMR15 CRISPR/Cas9 KO Plasmid (h) - sc-411633 from Santa Cruz Biotechnology
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MTMR15 HDR Plasmid (h) - sc-411633-HDR from Santa Cruz Biotechnology
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MTMR15 Double Nickase Plasmid (h) - sc-411633-NIC from Santa Cruz Biotechnology
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MTMR15 Double Nickase Plasmid (h2) - sc-411633-NIC-2 from Santa Cruz Biotechnology
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MTMR15 CRISPR Activation Plasmid (h) - sc-411633-ACT from Santa Cruz Biotechnology
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MTMR15 CRISPR Activation Plasmid (h2) - sc-411633-ACT-2 from Santa Cruz Biotechnology
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MTMR15 Lentiviral Activation Particles (h) - sc-411633-LAC from Santa Cruz Biotechnology
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MTMR15 Lentiviral Activation Particles (h2) - sc-411633-LAC-2 from Santa Cruz Biotechnology
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MTMR15 siRNA (h) - sc-90145 from Santa Cruz Biotechnology
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MTMR15 shRNA Plasmid (h) - sc-90145-SH from Santa Cruz Biotechnology
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MTMR15 shRNA (h) Lentiviral Particles - sc-90145-V from Santa Cruz Biotechnology
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MTMR15 RNAi - H00022909-R01 from Novus Biologicals
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MISSION® esiRNA - EHU126471 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN8470 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND8470 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR10250 from Sigma-Aldrich
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FAN1 siRNA (Human) - CRH7864 from Cohesion Biosciences
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FAN1 - MBS8227117 from MyBioSource
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shRNA set against Human FAN1 (NM_014967.4) - SHH292245 from Creative biogene
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Human FAN1 siRNA - orb268366 from Biorbyt
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FAN1 shRNA Plasmid (Human) - CHH7864 from Cohesion Biosciences
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FAN1 miRNA 3'UTR clone - MiUTR3H-12936 from Creative biogene
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FAN1 miRNA 3'UTR clone - MiUTR3H-12937 from Creative biogene
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FAN1 miRNA 3'UTR clone - MiUTR3H-12938 from Creative biogene
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FAN1 miRNA 3'UTR clone - MiUTR3H-12939 from Creative biogene
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