Gene Symbol | GAPDH |
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Entrez Gene | 2597 |
Alt Symbol | G3PD, GAPD, HEL-S-162eP |
Species | Human |
Gene Type | protein-coding |
Description | glyceraldehyde-3-phosphate dehydrogenase |
Other Description | aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH |
Swissprots | E7EUT4 Q53X65 P00354 P04406 |
Accessions | AAA53191 AAP88932 AEQ28035 AEQ28036 AEQ28037 EAW88786 EAW88787 P04406 AB062273 BAB93466 AF261085 AAF99678 AJ844644 CAH59755 AJ844645 CAH59756 AK026525 AK299448 BAG61421 AK308198 AY007133 AAG01996 AY633612 AAV33305 BC001601 AAH01601 BC004109 AAH04109 BC009081 AAH09081 BC013310 AAH13310 BC020308 BC023632 AAH23632 BC025925 AAH25925 BC026907 AAH26907 BC029340 BC029618 AAH29618 BC083511 AAH83511 BE893087 BG724119 BI463134 BM763361 BT006893 AAP35539 BU155402 CR407671 CAG28599 DQ403057 ABD77190 DQ891557 ABM82483 DQ894744 ABM85670 EF036498 ABO65084 EU668321 ACF94474 HQ727992 AET97565 HY000136 HY004110 HY022295 HY046784 J02642 AAA52496 M17851 AAA86283 M28283 AAA52519 M33197 AAA52518 M36164 X01677 CAA25833 X53778 CAA37794 NM_001256799 NP_001243728 NM_001289745 NP_001276674 NM_001289746 NP_001276675 NM_002046 NP_002037 |
Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibit |
Subcellular Location | Cytoplasm, cytosol {ECO:0000269|PubMed:12829261}. Nucleus {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:12829261}. Membrane {ECO:0000269|PubMed:12829261}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. {ECO:0000250}. |
Top Pathways | Glycolysis / Gluconeogenesis, Alzheimer's disease, Biosynthesis of amino acids, Carbon metabolism |
GAPDH CRISPR/Cas9 KO Plasmid (h) - sc-400002 from Santa Cruz Biotechnology
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GAPDH HDR Plasmid (h) - sc-400002-HDR from Santa Cruz Biotechnology
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GAPDH Double Nickase Plasmid (h) - sc-400002-NIC from Santa Cruz Biotechnology
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GAPDH Double Nickase Plasmid (h2) - sc-400002-NIC-2 from Santa Cruz Biotechnology
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GAPDH CRISPR Activation Plasmid (h) - sc-400002-ACT from Santa Cruz Biotechnology
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GAPDH CRISPR Activation Plasmid (h2) - sc-400002-ACT-2 from Santa Cruz Biotechnology
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GAPDH Lentiviral Activation Particles (h) - sc-400002-LAC from Santa Cruz Biotechnology
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GAPDH Lentiviral Activation Particles (h2) - sc-400002-LAC-2 from Santa Cruz Biotechnology
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GAPDH siRNA (h) - sc-35448 from Santa Cruz Biotechnology
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GAPDH shRNA Plasmid (h) - sc-35448-SH from Santa Cruz Biotechnology
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GAPDH shRNA (h) Lentiviral Particles - sc-35448-V from Santa Cruz Biotechnology
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GAPDH RNAi - H00002597-R01 from Novus Biologicals
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GAPDH RNAi - R0016 from Novus Biologicals
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MISSION® esiRNA - EHU146741 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2043 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2043 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR01430 from Sigma-Aldrich
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GAPDH siRNA (Human) - CRH1731 from Cohesion Biosciences
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GAPDH - MBS8220415 from MyBioSource
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shRNA set against Human GAPDH (NM_002046.4) - SHH299293 from Creative biogene
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GAPDH shRNA Plasmid (Human) - CHH1731 from Cohesion Biosciences
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GAPDH miRNA 3'UTR clone - MiUTR3H-00918 from Creative biogene
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