UBE2F

Gene Symbol UBE2F
Entrez Gene 140739
Alt Symbol NCE2
Species Human
Gene Type protein-coding
Description ubiquitin-conjugating enzyme E2F (putative)
Other Description NEDD8 carrier protein UBE2F|NEDD8 protein ligase UBE2F|NEDD8-conjugating enzyme 2|NEDD8-conjugating enzyme UBE2F
Swissprots B4DZU2 Q969M7 B4DMK3 H9KVB9 B8ZZG2 B4DDT9 C9J212 A8K1Z8 B4DFI1
Accessions AAY24220 CDM22106 EAW71128 EAW71129 EAW71130 EAW71131 EAW71132 EAW71133 EAW71134 Q969M7 AF310723 AAL26792 AK290063 BAF82752 AK293334 BAG56850 AK294107 BAG57442 AK297502 BAG59915 AK303094 BAG64204 BC010549 AAH10549 BC039435 BM470609 BM920749 BQ959565 BX421077 CN370973 CN370974 DA140546 DB118122 JF432618 ADZ15835 NM_001278305 NP_001265234 NM_001278306 NP_001265235 NM_001278307 NP_001265236 NM_001278308 NP_001265237 NM_080678 NP_542409 NR_103498 NR_103499 NR_103500
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. {ECO:0000269|PubMed:19250909}.
Tissue Specificity Widely expressed (at protein level). {ECO:0000269|PubMed:19250909}.
Top Pathways Ubiquitin mediated proteolysis

UBE2F (C-11) - sc-398668 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBE2F (C-11): sc-398668. Western blot analysis of UBE2F expression in non-transfected 293T: sc-117752 (A), mouse UBE2F transfected 293T: sc-124413 (B), Jurkat (C), HeLa (D), HL-60 (E) and NTERA-2 cl.D1 (F) whole cell lysates.

UBE2F (I-12) - sc-137891 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
UBE2F (I-12): sc-137891. Western blot analysis of UBE2F expression in non-transfected: sc-117752 (A) and mouse UBE2F transfected: sc-124413 (B) 293T whole cell lysates.

UBE2F (S-12) - sc-137893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
UBE2F (S-12): sc-137893. Western blot analysis of UBE2F expression in non-transfected: sc-110760 (A) and human UBE2F transfected: sc-112735 (B) 293 whole cell lysates.

UBE2F (C-23) - sc-130284 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
UBE2F (C-23): sc-130284. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.

Anti-NCE2 antibody - ab15707 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-NCE2 antibody (ab15707) at 1/1000 dilutionLane 1 : Endogenous Nce2 from mouse 3T3 cells Lane 2 : NIH 3T3 cells infected with ShRNA of mouse Nce2Lane 3 : NIH3T3 cells infected with ShRNA of Nce2 and MCSV-NCE2his flag-IRES-GFP (human NCE2)Lysates/proteins at 20 µg per lane.SecondaryHRP-conjugated rabbit polyclonal to goat IgGdeveloped using the ECL techniqueExposure time : 10 minutesSee Abreview

Anti-NCE2 antibody - ab38644 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NCE2 antibody (ab38644) + Jurkat cell lysate

Anti-NCE2 antibody [EPR12932] - ab185234 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12932
All lanes : Anti-NCE2 antibody [EPR12932] (ab185234) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : A375 cell lysateLane 3 : NTERA2/D1 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-UBE2F Antibody (aa152-164) - LS-C55644 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-UBE2F Antibody (N-Terminus) - LS-C81729 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-UBE2F Antibody (C-Terminus) - LS-C117234 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UBE2F / UBE2F antibody LS-C117234 Western blot of 293T cell lysate.

Anti-UBE2F Antibody (aa6-19) - LS-C139772 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
UBE2F antibody (0.05 ug/ml) staining of Rat Pancreas lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-UBE2F Antibody - LS-C155553 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE UBE2F antibody diluted at 1:1000

Anti-UBE2F Antibody (aa141-154) IHC-plus™ - LS-B6482 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-UBE2F antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6482 concentration 2.5 ug/ml.

UBE2F/NCE2 Antibody - NBP1-57602 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2F/NCE2 Antibody [NBP1-57602] - Sample Type: 1. Human NT-2 cells (60ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000

UBE2F/NCE2 Antibody - NBP1-92169 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NCE2 Antibody [NBP1-92169] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

UBE2F/NCE2 Antibody - NBP1-44062 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: UBE2F/NCE2 Antibody [NBP1-44062] - (0.05ug/ml) Analysis of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UBE2F/NCE2 Antibody - NB100-1151 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

UBE2F/NCE2 Antibody - NBP2-19292 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2F/NCE2 Antibody [NBP2-19292] - Analysis of UBE2F expression in transfected 293T cell line by UBE2F polyclonal antibody. A: Non-transfected lysate. B: UBE2F transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

UBE2F/NCE2 Antibody - NBP2-19485 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE2F/NCE2 Antibody [NBP2-19485] - Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE gel, diluted at 1:1000.

NCE2 Polyclonal Antibody - bs-8378R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE2F antibody produced in rabbit - AV52515 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: HeLa (<B>Cat. No. AV52515</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1 μg/mL in cell type HeLa

Anti-UBE2F (N-terminal) antibody produced in goat - SAB2501889 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-UBE2F (N-terminal): <B>Cat. No. SAB2501889</B>: (0.05 μg/mL) staining of Rat Pancreas lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-UBE2F (C-terminal) antibody produced in goat - SAB2501197 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-UBE2F (C-terminal): <B>Cat. No. SAB2501197</B>: (0.05 μg/mL) staining of Mouse Liver lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UBE2F antibody - N-terminal region (ARP52515_P050) - ARP52515_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

UBE2F antibody - middle region (ARP52516_P050) - ARP52516_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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