KRIT1

Gene Symbol	KRIT1
Entrez Gene	889
Alt Symbol	CAM, CCM1
Species	Human
Gene Type	protein-coding
Description	KRIT1, ankyrin repeat containing
Other Description	ankyrin repeat-containing protein Krit1\|cerebral cavernous malformations 1 protein\|krev interaction trapped 1\|krev interaction trapped protein 1
Swissprots	Q506L6 Q9H180 O00522 O43894 Q75N19 A6NNU0 Q9HAX5 Q6U276 Q9H264
Accessions	AAC01535 AAS07420 AAY25568 EAL24152 EAW76854 EAW76855 O00522 AF296765 AAG10220 AF310133 AAG47774 AF388384 AAM19465 AJ294850 CAC17608 AK055305 BAG51497 AK056537 BAG51741 AK226131 AL049325 AY380057 AAQ94072 AY380058 AAQ94073 AY380059 AAQ94074 AY380060 AAQ94075 AY993944 AAY25567 BC094684 AAH94684 BQ070587 BX648659 U90268 AAB58582 XM_005250660 XP_005250717 XM_005250662 XP_005250719 XM_005250664 XP_005250721 XM_005250665 XP_005250722 XM_005250666 XP_005250723 XM_005250667 XP_005250724 XM_005250668 XP_005250725 XM_005250669 XP_005250726 XM_006716160 XP_006716223 XM_006716161 XP_006716224 XM_006716162 XP_006716225 XM_006716163 XP_006716226 XM_011516651 XP_011514953 XM_011516652 XP_011514954 XM_011516653 XP_011514955 XM_011516654 XP_011514956 XM_011516655 XP_011514957 XM_011516656 XP_011514958 XM_011516657 XP_011514959 XM_011516658 XP_011514960 XM_011516659 XP_011514961 XM_011516660 XP_011514962 XM_011516661 XP_011514963 NM_001013406 NP_001013424 NM_004912 NP_004903 NM_194454 NP_919436 NM_194455 NP_919437 NM_194456 NP_919438
Function	Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity). Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule- associated protein that binds to phosphatidylinositol 4,5
Subcellular Location	Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Cell junction. Note=KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. Association with RAP1 relocalizes KRIT1 from microtubules to cell junction membranes. Translocates from the cytoplasm along microtubules to the cell membrane in a ITGB1BP1- dependent manner.
Tissue Specificity	Low levels in brain. Very weak expression found in heart and muscle. {ECO:0000269\|PubMed:9285558}.
Top Pathways	Rap1 signaling pathway

KRIT1 (E-8) - sc-514371 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

KRIT1 (E-8): sc-514371. Western blot analysis of KRIT1 expression in Raji (A) and SH-SY5Y (B) whole cell lysates.

KRIT1 (H-262) - sc-367533 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

KRIT1 (K-16) - sc-23997 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

KRIT1 (K-16): sc-23997. Western blot analysis of KRIT1 expression in IMR-32 whole cell lysate (A) and mouse brain tissue extract (B).

KRIT1 (8-RY2) - sc-134376 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

KRIT1 (8-RY2): sc-134376. Western blot analysis of KRIT1 expression in human KRIT1 transfected (A) and non-transfected (B) 293T whole cell lysates.

Anti-KRIT1 antibody - ab110530 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-KRIT1 antibody (ab110530) at 1 µg/mlLane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-KRIT1 antibody - ab111504 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescent staining of Human foreskin, using ab111504 at a 1/50 dilution (red). Costaining of endothelial cells is with anti CD31 (green). Note specific staining in the wall of a subset of vessel. Nuclei are counter-stained with Dapi (blue).

Anti-KRIT1 antibody [EPR16560] - ab196025 from Abcam

Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR16560

All lanes : Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilutionLane 1 : Human fetal brain lysateLane 2 : SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysateLane 3 : Raji (Human Burkitt's lymphoma cell line) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-CCM1 / KRIT1 Antibody (aa6-34, AP) - LS-C236925 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa6-34, FITC) - LS-C236927 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa6-34, Biotin) - LS-C236926 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa6-34, PE) - LS-C236929 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa6-34, APC) - LS-C236928 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa5-34) - LS-C322549 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa6-34, Azide-free, HRP) - LS-C248668 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CCM1 / KRIT1 Antibody (aa637-737, Azide-free) - LS-C327732 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal

Anti-CCM1 / KRIT1 Antibody (clone 2C7) - LS-C196831 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2C7

Detection limit for recombinant GST tagged KRIT1 is approximately 0.03 ng/ml as a capture antibody.

Anti-CCM1 / KRIT1 Antibody (aa5-34) - LS-C160019 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

KRIT1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The KRIT1 antibody detected the KRIT1 protein (arrow).

Anti-CCM1 / KRIT1 Antibody - LS-C346127 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence blot of HeLa cell using KRIT1 antibody. Blue: DAPI for nuclear staining.

KRIT1 Antibody - NBP2-19307 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: KRIT1 Antibody [NBP2-19307] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.

KRIT1 Antibody (2C7) - H00000889-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2C7

KRIT1 Antibody - H00000889-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

KRIT1 Antibody (1D8) - H00000889-M02 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1D8

Sandwich ELISA: KRIT1 Antibody (1D8) [H00000889-M02] - Detection limit for recombinant GST tagged KRIT1 is 0.1 ng/ml as a capture antibody.

KRIT1 Polyclonal Antibody - bs-8546R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

$Antigen: bs-8546P, 0.2ug/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Monoclonal Anti-KRIT1, (C-terminal) antibody produced in mouse - SAB1403643 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1D8

<B>ELISA</B><BR/>Monoclonal Anti-KRIT1: <B>Cat. No. SAB1403643</B>: Detection limit for recombinant GST tagged KRIT1 is 0.1 ng/mL.

Anti-KRIT1 antibody produced in rabbit - HPA049606 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-KRIT1: <B>Cat. No. HPA049606</B>: Immunohistochemical staining of human small intestine shows distinct positivity in a subset of vessels.

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